NNMT Antikörper (AA 8-264)

Produktnummer ABIN7876814

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NNMT in WB, ELISA, IF, IP und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NNMT Antikörper (AA 8-264) (ABIN7876814)

Target: NNMT (Nicotinamide N-Methyltransferase (NNMT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NNMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-NNMT Antikörper

Bindungsspezifität: AA 8-264

Verwendungszweck: NNMT Antibody / Nicotinamide N-methyltransferase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NNMT recombinant protein (Position: K8-L264) was used as the immunogen for the NNMT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NNMT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NNMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NNMT

Target: NNMT (Nicotinamide N-Methyltransferase (NNMT))

Andere Bezeichnung: NNMT

Hintergrund: The NNMT antibody targets Nicotinamide N-methyltransferase, a cytosolic enzyme encoded by the NNMT gene that catalyzes the methylation of nicotinamide to form 1-methylnicotinamide (1-MNA) using S-adenosyl-L-methionine (SAM) as a methyl donor. This enzyme plays an essential role in nicotinamide metabolism and methyl group utilization, linking energy homeostasis with epigenetic regulation. Nicotinamide N-methyltransferase influences cellular NAD+ turnover, methylation capacity, and energy balance. The NNMT antibody enables detailed investigation of this metabolic enzyme in physiology, cancer, and metabolic disease.

Nicotinamide N-methyltransferase is expressed in various tissues, with the highest levels in liver, adipose tissue, and skeletal muscle. By regulating nicotinamide levels, NNMT indirectly affects sirtuin activity and NAD+-dependent deacetylation pathways. The NNMT antibody supports research into how this enzyme modulates mitochondrial function, stress resistance, and cellular metabolism. Its detection across metabolic tissues allows mapping of expression patterns that correlate with nutrient availability and hormonal regulation.

Dysregulation of NNMT expression is associated with multiple diseases. Overexpression promotes tumorigenesis by altering cellular methyl donor availability and epigenetic modifications. Elevated Nicotinamide N-methyltransferase activity has been observed in cancers of the liver, kidney, and pancreas, where it contributes to proliferation and metastasis. Conversely, reduced NNMT activity has been linked to insulin resistance and altered lipid metabolism. The NNMT antibody is widely used to quantify enzyme levels and evaluate its role in metabolic reprogramming, energy balance, and disease progression.

In metabolic research, Nicotinamide N-methyltransferase is recognized as a master regulator of one-carbon metabolism, influencing the methionine cycle and SAM/SAH ratio. The NNMT antibody enables functional studies exploring how modulation of enzyme activity impacts methylation reactions and gene expression. Additionally, NNMT-derived metabolites such as 1-MNA exhibit signaling functions that affect endothelial function and anti-inflammatory responses, broadening the enzyme's physiological relevance.

NSJ Bioreagents supplies the NNMT antibody as a validated reagent for western blotting, immunohistochemistry, and immunofluorescence. It provides consistent, high-specificity detection of Nicotinamide N-methyltransferase across tissues and experimental systems. By supporting research into metabolism, oncology, and epigenetics, the NNMT antibody helps elucidate how methyltransferase regulation integrates cellular energy metabolism with gene expression and disease susceptibility.

UniProt: P40261