NUP43 Antikörper (AA 76-380)

Produktnummer ABIN7876747

Der Kaninchen Polyklonal anti-NUP43 Antikörper (ABIN7876747) detektiert spezifisch NUP43 in WB, ELISA, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für NUP43 Antikörper (AA 76-380) (ABIN7876747)

Target: NUP43 (Nucleoporin 43kDa (NUP43))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUP43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NUP43 Antikörper

Bindungsspezifität: AA 76-380

Verwendungszweck: NUP43 Antibody / Nucleoporin 43

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NUP43 recombinant protein (Position: D76-S380) was used as the immunogen for the NUP43 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUP43 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUP43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUP43

Target: NUP43 (Nucleoporin 43kDa (NUP43))

Andere Bezeichnung: NUP43

Hintergrund: NUP43 antibody detects Nuclear pore complex protein Nup43, encoded by the NUP43 gene on chromosome 6p21.33. NUP43 antibody is widely used in studies of nucleocytoplasmic transport, nuclear pore structure, and gene regulation. NUP43 is one of the small, conserved subunits of the nuclear pore complex (NPC), a massive macromolecular assembly that regulates bidirectional transport of RNA, proteins, and signaling molecules across the nuclear envelope. By interacting with scaffold nucleoporins, NUP43 helps stabilize the Y-complex, a structural subassembly of the NPC.

Structurally, NUP43 is a ~43 kDa protein with a WD40 beta-propeller domain that mediates protein-protein interactions. It interacts with NUP107, NUP133, and other Y-complex components to form a stable subcomplex essential for pore assembly. NUP43 localization is restricted to the nuclear envelope, consistent with its structural role in NPC architecture. Its highly conserved nature across species emphasizes its importance in nuclear transport machinery.

Functionally, NUP43 supports NPC assembly, nuclear import and export, and chromatin organization. During mitosis, the Y-complex including NUP43 contributes to reformation of the nuclear envelope after breakdown. Loss of NUP43 destabilizes the Y-complex, impairing nuclear transport and genome organization. Researchers use NUP43 antibody to study nuclear pore biology, gene expression regulation, and nuclear architecture.

Clinically, NUP43 has been linked to cancer and developmental disorders. Altered expression and mutations in nucleoporins, including NUP43, are associated with leukemias and solid tumors, where transport dysregulation promotes abnormal signaling. Variants in NUP43 have also been implicated in neurodevelopmental disorders due to impaired nuclear-cytoplasmic communication. NSJ Bioreagents provides NUP43 antibody for use in nuclear transport, cancer, and developmental biology research.

Experimentally, NUP43 antibody is used in western blotting to detect the ~43 kDa protein, in immunofluorescence to highlight nuclear envelope localization, and in immunohistochemistry to analyze expression in tissue sections. Co-immunoprecipitation with NUP43 antibody identifies interaction partners within the NPC Y-complex.

UniProt: Q8NFH3