RAB1A Antikörper (AA 74-199)

Produktnummer ABIN7876703

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RAB1A in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für RAB1A Antikörper (AA 74-199) (ABIN7876703)

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-RAB1A Antikörper

Bindungsspezifität: AA 74-199

Verwendungszweck: RAB1A Antibody / Ras-related protein Rab-1A

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RAB1A recombinant protein (Position: R74-Q199) was used as the immunogen for the RAB1A antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RAB1A antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RAB1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RAB1A

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Andere Bezeichnung: RAB1A

Hintergrund: RAB1A antibody detects Ras-related protein Rab-1A, a small GTPase involved in vesicular trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. The UniProt recommended name is Ras-related protein Rab-1A (RAB1A). This essential member of the Rab family regulates the formation, movement, and docking of transport vesicles that ensure protein secretion and Golgi organization.

Functionally, RAB1A antibody identifies a 205-amino-acid GTP-binding protein that cycles between active GTP-bound and inactive GDP-bound states. Active RAB1A recruits effector proteins such as p115, GM130, and COPII components to mediate vesicle tethering and fusion at the cis-Golgi membrane. It coordinates early secretory pathway events and maintains ER-to-Golgi transport fidelity, supporting proper protein maturation and trafficking.

The RAB1A gene is located on chromosome 2p14 and is ubiquitously expressed in eukaryotic cells, with particularly high expression in secretory tissues. Its activity is regulated by GEFs (guanine nucleotide exchange factors) and GAPs (GTPase-activating proteins) that control the GTP/GDP cycle and subcellular localization.

Pathologically, RAB1A dysregulation affects autophagy, neurodegeneration, and cancer progression. Overexpression enhances cell proliferation and Golgi reorganization in tumor cells, while impaired RAB1A function contributes to ER stress and defective autophagosome formation. Research using RAB1A antibody supports studies in membrane trafficking, Golgi function, and oncogenic signaling.

RAB1A antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect small GTPases. NSJ Bioreagents provides RAB1A antibody reagents optimized for research in vesicle transport, protein secretion, and cellular trafficking dynamics.

Structurally, Ras-related protein Rab-1A contains conserved GTP-binding motifs (GxxxxGK[S/T], DxxG, NKxD, and SAx) and a C-terminal geranylgeranylation site that anchors it to membranes. Its switch regions undergo conformational changes upon GTP binding, enabling interaction with effectors that mediate vesicle transport. This antibody allows exploration of RAB1A's role in ER-Golgi trafficking and its contribution to secretory pathway regulation.

UniProt: P62820

Pathways: SARS-CoV-2 Protein Interaktom