NUP98 Antikörper (AA 728-1787)

Produktnummer ABIN7876646

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NUP98 in WB, ELISA, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Affe.

Kurzübersicht für NUP98 Antikörper (AA 728-1787) (ABIN7876646)

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUP98 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NUP98 Antikörper

Bindungsspezifität: AA 728-1787

Verwendungszweck: NUP98 Antibody / Nucleoporin 98

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NUP98 recombinant protein (Position: H728-I1787) was used as the immunogen for the NUP98 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUP98 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUP98 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUP98

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Andere Bezeichnung: NUP98

Hintergrund: NUP98 antibody detects Nuclear pore complex protein Nup98, a critical nucleoporin involved in nucleocytoplasmic transport, gene regulation, and mitotic progression. Encoded by the NUP98 gene on chromosome 11p15.4, this large modular protein forms part of the nuclear pore complex (NPC), which mediates bidirectional transport of macromolecules between the nucleus and cytoplasm. Nup98 functions not only as a structural component of the NPC but also as a dynamic regulator of gene expression and chromatin organization.

Nup98 contains FG (phenylalanine-glycine)-repeat domains that form flexible meshwork structures facilitating selective nucleocytoplasmic transport. The C-terminal region anchors to the NPC scaffold via interactions with other nucleoporins such as Nup96 and Nup88. Beyond its classical transport role, Nup98 shuttles between the nuclear envelope and nucleoplasm, where it binds transcriptional regulators, influencing developmental and immune response genes. It also participates in RNA export, mRNA surveillance, and mitotic spindle assembly.

The NUP98 antibody is widely used in cell biology, oncology, and molecular genetics research to study nuclear transport, chromatin organization, and cell cycle regulation. Western blot analysis identifies a 98 kilodalton band corresponding to Nup98, while immunofluorescence shows punctate staining at the nuclear envelope and within nucleoplasmic foci. This antibody supports studies examining NPC function, transcriptional regulation, and nuclear architecture integrity.

Translocations involving NUP98 are recurrent in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), producing oncogenic Nup98 fusion proteins such as NUP98-HOXA9 and NUP98-NSD1. These fusions retain the FG-repeat domain of Nup98 but acquire transcriptional activation capabilities from their fusion partners, leading to deregulated gene expression and leukemogenesis. The NUP98 antibody provides a reliable tool for studying such fusion proteins and their oncogenic mechanisms. NSJ Bioreagents validates this antibody for its applications, ensuring accurate detection for nuclear transport and cancer research.

UniProt: P52948

Pathways: Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interaktom