FGD5 Antikörper (AA 705-1462)

Produktnummer ABIN7876587

Der Kaninchen Polyklonal anti-FGD5 Antikörper wird verwendet zum Nachweis von FGD5 in Proben von Human, Maus und Ratte. Er wurde validiert für ELISA, WB und FACS.

Kurzübersicht für FGD5 Antikörper (AA 705-1462) (ABIN7876587)

Target: FGD5 (FYVE, RhoGEF and PH Domain Containing 5 (FGD5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGD5 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-FGD5 Antikörper

Bindungsspezifität: AA 705-1462

Verwendungszweck: FGD5 Antibody / FYVE, RhoGEF and PH domain-containing protein 5

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human FGD5 recombinant protein (Position: Q705-L1462) was used as the immunogen for the FGD5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the FGD5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the FGD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FGD5

Target: FGD5 (FYVE, RhoGEF and PH Domain Containing 5 (FGD5))

Andere Bezeichnung: FGD5

Hintergrund: The FGD5 antibody targets FYVE, RhoGEF, and PH domain-containing protein 5, a guanine nucleotide exchange factor encoded by the FGD5 gene. This protein belongs to the FGD family of Rho guanine nucleotide exchange factors (GEFs) that activate CDC42, a small GTPase involved in cytoskeletal organization, vesicle trafficking, and angiogenesis. FYVE, RhoGEF, and PH domain-containing protein 5 is enriched in endothelial cells and plays a critical role in vascular development and remodeling. The FGD5 antibody enables detection and quantification of this signaling molecule, supporting research into endothelial cell biology and vascular homeostasis.

FGD5 contains multiple conserved domains, including a FYVE domain that binds phosphatidylinositol 3-phosphate, a Dbl homology (DH) domain that catalyzes GTP loading of CDC42, and a pleckstrin homology (PH) domain that anchors the protein to membranes. These domains coordinate spatial activation of CDC42 at specific cellular sites, promoting actin cytoskeletal rearrangements necessary for cell migration and tube formation. The FGD5 antibody provides a key reagent for examining how this protein localizes to endosomes and plasma membrane regions during angiogenic signaling.

Endothelial FGD5 regulates cell polarity, barrier integrity, and lumen formation by activating CDC42 and downstream effectors such as PAK and N-WASP. Its expression is induced by vascular growth factors, including VEGF and FGF, which drive endothelial morphogenesis. The FGD5 antibody supports studies into these processes, helping delineate how Rho GTPase signaling orchestrates angiogenic sprouting and vessel stabilization. Loss of FGD5 function leads to impaired vascular network formation, highlighting its essential developmental role.

Beyond angiogenesis, FYVE, RhoGEF, and PH domain-containing protein 5 contributes to intracellular trafficking and signal transduction in other tissues, including kidney and brain. Abnormal FGD5 expression has been linked to vascular pathologies, diabetic retinopathy, and cancer progression, where altered endothelial behavior supports tumor angiogenesis. The FGD5 antibody allows researchers to quantify expression changes under pathological conditions and explore its regulatory influence on endothelial plasticity.

The FGD5 antibody performs well in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and membrane-associated staining. NSJ Bioreagents provides this antibody as a validated reagent for consistent performance across model systems. By facilitating detailed analysis of FYVE, RhoGEF, and PH domain-containing protein 5 expression and function, the FGD5 antibody supports vascular biology research and contributes to understanding the molecular mechanisms driving angiogenesis and endothelial cell dynamics.

UniProt: Q6ZNL6