NSDHL Antikörper (AA 7-373)

Produktnummer ABIN7876491

Der Kaninchen Polyklonal anti-NSDHL Antikörper wird verwendet zum Nachweis von NSDHL in Proben von Human, Ratte und Maus. Er wurde validiert für WB, ELISA, IHC, IF, IP und FACS.

Kurzübersicht für NSDHL Antikörper (AA 7-373) (ABIN7876491)

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSDHL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-NSDHL Antikörper

Bindungsspezifität: AA 7-373

Verwendungszweck: NSDHL Antibody / Sterol-4-alpha-carboxylate 3-dehydrogenase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NSDHL recombinant protein (Position: E7-K373) was used as the immunogen for the NSDHL antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NSDHL antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NSDHL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NSDHL

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Andere Bezeichnung: NSDHL

Hintergrund: NSDHL antibody detects Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, a membrane-associated enzyme involved in cholesterol biosynthesis. The UniProt recommended name is Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL). This enzyme catalyzes oxidative decarboxylation of sterol intermediates during the post-squalene steps of cholesterol production, playing a crucial role in lipid homeostasis and membrane integrity.

Functionally, NSDHL antibody identifies a 373-amino-acid enzyme localized to the endoplasmic reticulum membrane. NSDHL acts sequentially with other enzymes such as HSD17B7 and EBP to remove carboxyl groups from C4-methyl sterols, facilitating the conversion of lanosterol to cholesterol. Its catalytic activity contributes to maintaining sterol balance essential for cell signaling and membrane function.

The NSDHL gene is located on chromosome Xq28 and is highly expressed in liver, skin, and brain tissues. Its activity supports embryonic development, epidermal differentiation, and lipid raft formation in cell membranes. NSDHL is evolutionarily conserved and tightly regulated to ensure steady cholesterol output.

Pathologically, mutations in NSDHL cause CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects), a rare X-linked disorder involving defective cholesterol metabolism and asymmetric skin and limb malformations. NSDHL dysfunction also disrupts sterol intermediates, leading to toxic accumulation of precursors. Research using NSDHL antibody supports studies in lipid metabolism, cholesterol biosynthesis, and genetic disorders of sterol processing.

NSDHL antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sterol biosynthetic enzymes. NSJ Bioreagents provides NSDHL antibody reagents optimized for lipid biology, metabolism, and enzymology research.

Structurally, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating contains a short-chain dehydrogenase/reductase catalytic domain and transmembrane segments that anchor it to the endoplasmic reticulum. This antibody enables study of NSDHL's role in sterol conversion and metabolic regulation.

UniProt: Q15738