NCOA6 Antikörper (AA 64-1475)

Produktnummer ABIN7876250

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NCOA6 in ELISA, WB, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NCOA6 Antikörper (AA 64-1475) (ABIN7876250)

Target: NCOA6 (Nuclear Receptor Coactivator 6 (NCOA6))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOA6 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-NCOA6 Antikörper

Bindungsspezifität: AA 64-1475

Verwendungszweck: NCOA6 Antibody / Nuclear receptor coactivator 6

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NCOA6 recombinant protein (Position: K64-E1475) was used as the immunogen for the NCOA6 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NCOA6 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NCOA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NCOA6

Target: NCOA6 (Nuclear Receptor Coactivator 6 (NCOA6))

Andere Bezeichnung: NCOA6

Hintergrund: NCOA6 antibody detects Nuclear receptor coactivator 6, a transcriptional coactivator that enhances the activity of nuclear hormone receptors and other transcription factors. Encoded by the NCOA6 gene on chromosome 20q12, this large nuclear protein plays a central role in gene expression regulation, chromatin remodeling, and hormone signaling. NCOA6, also known as ASC-2 (Activator of Steroid Receptor Coactivator-2), functions within multiprotein complexes that recruit histone acetyltransferases (HATs) and methyltransferases to promote transcriptional activation. Its modular structure includes LXXLL nuclear receptor-interaction motifs, a central activation domain, and regions that associate with chromatin modifiers such as CBP/p300 and CARM1.

NCOA6 acts as a transcriptional bridge connecting ligand-activated nuclear receptors-including estrogen receptor, thyroid hormone receptor, glucocorticoid receptor, and peroxisome proliferator-activated receptor gamma (PPARG)-to the general transcriptional machinery. Through these interactions, NCOA6 antibody detects a coactivator that is critical for endocrine signaling and metabolism regulation. NCOA6 also cooperates with non-receptor transcription factors such as E2F1, p53, and CREB-binding protein, extending its role beyond hormonal pathways. In metabolic tissues, it contributes to lipid metabolism, gluconeogenesis, and energy homeostasis through regulation of PGC-1alpha and FOXO1 signaling networks.

Structurally, NCOA6 contains multiple protein-binding domains that facilitate interaction with chromatin-modifying enzymes and transcriptional complexes. It functions as a scaffold for the ASCOM complex (ASC-2 complex) that includes MLL family methyltransferases responsible for histone H3 lysine 4 (H3K4) methylation-a mark of active transcription. These modifications alter chromatin accessibility and promote RNA polymerase II recruitment. Alternative splicing of NCOA6 produces several isoforms with tissue-specific regulatory effects.

NCOA6 is expressed ubiquitously but is enriched in liver, reproductive tissues, and endocrine organs. Dysregulation of NCOA6 has been linked to developmental abnormalities, reproductive disorders, and cancers including breast, prostate, and liver carcinoma. Overexpression promotes estrogen-dependent gene transcription and can contribute to hormone-sensitive tumor progression. Genetic studies have also implicated NCOA6 variants in metabolic syndrome, insulin resistance, and congenital heart defects, reflecting its broad physiological significance.

At the chromosomal level, 20q12 amplification encompassing NCOA6 has been identified in several tumor types. Functionally, NCOA6 acts as a coactivator of MYC and E2F transcription factors, enhancing cell proliferation and oncogenic transformation. Conversely, NCOA6 depletion impairs cell-cycle progression and induces apoptosis, indicating its importance for tumor cell survival. Disease-relevant pathways regulated by NCOA6 include oxidative stress response, estrogen signaling, and retinoic acid receptor pathways.

Immunohistochemical staining using NCOA6 antibody shows nuclear localization in hepatocytes, endocrine gland cells, and epithelial tissues. The antibody is a valuable tool for studies of transcriptional regulation, hormone receptor signaling, and chromatin remodeling. NCOA6 antibody from NSJ Bioreagents provides specific detection of this multifunctional nuclear coactivator for research in endocrinology, oncology, and molecular biology.

UniProt: Q14686

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha