POC1A Antikörper (AA 6-371)

Produktnummer ABIN7875960

Der Kaninchen Polyklonal anti-POC1A Antikörper wird verwendet zum Nachweis von POC1A in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA, IF, FACS und ICC.

Kurzübersicht für POC1A Antikörper (AA 6-371) (ABIN7875960)

Target: POC1A (POC1 Centriolar Protein Homolog A (POC1A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POC1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-POC1A Antikörper

Bindungsspezifität: AA 6-371

Verwendungszweck: POC1A Antibody / POC1 centriolar protein A

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human POC1A recombinant protein (Position: A6-D371) was used as the immunogen for the POC1A antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the POC1A antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the POC1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu POC1A

Target: POC1A (POC1 Centriolar Protein Homolog A (POC1A))

Andere Bezeichnung: POC1A

Hintergrund: POC1A antibody detects POC1 centriolar protein A, a core structural component of centrioles essential for their assembly, stability, and duplication. POC1A belongs to a conserved family of centriolar proteins that maintain centrosome integrity and regulate spindle formation during mitosis. The POC1A antibody is widely used in cell cycle, ciliogenesis, and developmental biology research to study centrosome organization and division control.

POC1A is encoded by the POC1A gene located on human chromosome 3p21.2. The protein is approximately 45 kilodaltons and contains an N-terminal WD40 domain that mediates protein-protein interactions and a C-terminal coiled-coil region required for centriolar localization. POC1A localizes to the proximal ends of centrioles and basal bodies, where it contributes to centriole duplication fidelity and stability.

The POC1A antibody detects a 45 kilodalton band in western blot assays and shows centrosomal staining in interphase and mitotic cells. POC1A is required for the maintenance of mature centrioles and proper spindle orientation during cell division. It works in concert with other centriolar components, including CEP135 and SAS6, ensuring correct microtubule organization and ciliogenesis.

Mutations in POC1A cause primordial dwarfism and growth retardation syndromes due to defective centriole biogenesis and impaired mitotic progression. Cells lacking POC1A exhibit abnormal centrosome numbers, fragmented spindles, and cell cycle arrest. POC1A expression is also implicated in cilia assembly, linking centrosome function to signaling pathways such as Hedgehog and Wnt.

Because of its importance in centrosome biology, POC1A serves as a useful marker for studying mitotic structures, developmental abnormalities, and ciliopathies. NSJ Bioreagents provides a validated POC1A antibody optimized for western blot, immunofluorescence, and centrosome imaging, supporting research into centriole duplication, ciliary formation, and cell division regulation.

UniProt: Q8NBT0