TFF1 Antikörper (AA 57-84)

Produktnummer ABIN7875814

Der Maus Monoklonal anti-TFF1 Antikörper (Klon SPM573) (ABIN7875814) detektiert spezifisch TFF1 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TFF1 Antikörper (AA 57-84) (ABIN7875814)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TFF1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM573

Produktdetails anti-TFF1 Antikörper

Bindungsspezifität: AA 57-84

Verwendungszweck: pS2 Antibody / TFF1

Sequenz: CFDDTVRGVP WCFYPNTIDV PPEEECEF

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 57-84 (CFDDTVRGVPWCFYPNTIDVPPEEECEF) from the human protein were used as the immunogen for this pS2 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the pS2 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the pS2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Andere Bezeichnung: pS2

Hintergrund: It recognizes a polypeptide of 6.5 kDa, identified as pS2 estrogen-regulated protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

UniProt: P04155

Pathways: EGFR Signaling Pathway