SMYD1 Antikörper (AA 57-472)

Produktnummer ABIN7875807

Der Kaninchen Polyklonal anti-SMYD1 Antikörper wird verwendet zum Nachweis von SMYD1 in Proben von Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für SMYD1 Antikörper (AA 57-472) (ABIN7875807)

Target: SMYD1 (SET and MYND Domain Containing 1 (SMYD1))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMYD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SMYD1 Antikörper

Bindungsspezifität: AA 57-472

Verwendungszweck: SMYD1 Antibody / SET and MYND domain-containing protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SMYD1 recombinant protein (Position: K57-Q472) was used as the immunogen for the SMYD1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SMYD1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SMYD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SMYD1

Target: SMYD1 (SET and MYND Domain Containing 1 (SMYD1))

Andere Bezeichnung: SMYD1

Hintergrund: SMYD1 antibody detects SET and MYND domain-containing protein 1, a muscle-specific histone methyltransferase that regulates gene expression during cardiac and skeletal muscle differentiation. SMYD1 modulates transcription by methylating histone H3 on lysine 4 and recruiting transcriptional cofactors to promoter regions of muscle-specific genes. The SMYD1 antibody is widely used in developmental biology and epigenetics research to study muscle gene regulation, histone modification, and cardiogenesis.

SMYD1 is encoded by the SMYD1 gene located on human chromosome 2p11.2. The protein is approximately 490 amino acids long and contains an N-terminal SET domain responsible for methyltransferase activity and a C-terminal MYND zinc-finger domain that mediates protein-protein interactions. SMYD1 is predominantly expressed in heart and skeletal muscle tissues, where it coordinates transcriptional programs required for myofibril assembly and contractile function.

The SMYD1 antibody detects a 55 kilodalton band by western blot and shows nuclear and sarcoplasmic staining under immunofluorescence microscopy. SMYD1 acts as a transcriptional regulator of genes encoding structural and contractile proteins such as myosin heavy chains and troponins. It interacts with cofactors like HDACs and skNAC to coordinate repression and activation cycles during myogenesis.

Loss of SMYD1 function in animal models results in defective cardiac morphogenesis, reduced sarcomere organization, and embryonic lethality, highlighting its critical developmental role. Dysregulation of SMYD1 expression has been linked to cardiomyopathy and muscular dystrophy, where abnormal histone methylation alters muscle gene networks. Beyond muscle, SMYD1 may influence differentiation of smooth muscle and endothelial lineages through epigenetic signaling.

Because SMYD1 couples chromatin modification to tissue-specific transcription, it represents a pivotal regulator of muscle differentiation and cardiac development. NSJ Bioreagents provides a validated SMYD1 antibody optimized for its applications, supporting research into epigenetic control, muscle biology, and developmental gene regulation.

UniProt: Q8NB12

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development