GIGYF1 Antikörper (AA 55-950)

Produktnummer ABIN7875703

Der Kaninchen Polyklonal anti-GIGYF1 Antikörper (ABIN7875703) detektiert spezifisch GIGYF1 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GIGYF1 Antikörper (AA 55-950) (ABIN7875703)

Target: GIGYF1 (GRB10 Interacting GYF Protein 1 (GIGYF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GIGYF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-GIGYF1 Antikörper

Bindungsspezifität: AA 55-950

Verwendungszweck: GIGYF1 Antibody / GRB10-interacting GYF protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human GIGYF1 recombinant protein (Position: K55-E950) was used as the immunogen for the GIGYF1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GIGYF1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GIGYF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GIGYF1

Target: GIGYF1 (GRB10 Interacting GYF Protein 1 (GIGYF1))

Andere Bezeichnung: GIGYF1

Hintergrund: GIGYF1 antibody detects GRB10-interacting GYF protein 1, encoded by the GIGYF1 gene on chromosome 7q22. GIGYF1 antibody is widely applied in studies of insulin signaling, mRNA regulation, and cell growth. GIGYF1 interacts with GRB10, an adaptor protein in insulin and growth factor receptor pathways, modulating downstream signaling. It also participates in post-transcriptional regulation by binding mRNA cap-binding proteins and translational regulators. Expression is detected in multiple tissues, including brain, pancreas, and muscle, consistent with its roles in metabolism and growth control.

Structurally, GIGYF1 is a protein containing a GYF domain, which recognizes proline-rich motifs, and regions mediating interactions with GRB10 and mRNA-binding proteins. Alternative splicing produces isoforms with variable interaction capacity. Its dual role in signaling and RNA biology reflects structural versatility.

Functionally, GIGYF1 acts as a scaffold linking GRB10 to insulin receptor pathways, regulating PI3K-Akt and MAPK cascades. It also partners with 4EHP, a cap-binding protein, to repress translation of specific mRNAs. Through these mechanisms, GIGYF1 influences metabolism, cell proliferation, and stress responses. Researchers use GIGYF1 antibody to study insulin signaling, translational repression, and metabolic control.

Clinically, GIGYF1 has been implicated in diabetes, neurodevelopmental disorders, and cancer. Genetic variants in GIGYF1 are associated with type 2 diabetes risk and altered glucose homeostasis. Loss-of-function mutations have been linked to developmental delay, autism, and epilepsy, highlighting roles in brain function. Dysregulated expression contributes to oncogenesis, where altered receptor signaling drives proliferation. NSJ Bioreagents supplies GIGYF1 antibody to support studies in endocrinology, neurology, and oncology.

Experimentally, GIGYF1 antibody is used in western blotting to detect the ~160 kDa protein, in immunofluorescence to study subcellular localization, and in immunohistochemistry to evaluate tissue-specific expression. Co-immunoprecipitation with GIGYF1 antibody identifies complexes containing GRB10, 4EHP, and translational regulators.

UniProt: O75420