LRRC75A/FAM211A Antikörper (AA 52-292)

Produktnummer ABIN7875558

Der Kaninchen Polyklonal anti-LRRC75A/FAM211A Antikörper wird verwendet zum Nachweis von LRRC75A/FAM211A in Proben von Human. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für LRRC75A/FAM211A Antikörper (AA 52-292) (ABIN7875558)

Target: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LRRC75A/FAM211A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-LRRC75A/FAM211A Antikörper

Bindungsspezifität: AA 52-292

Verwendungszweck: LRRC75A Antibody / FAM211A

Aufreinigung: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids H52-Q292) was used as the immunogen for the LRRC75A antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LRRC75A antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LRRC75A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LRRC75A/FAM211A

Target: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Andere Bezeichnung: LRRC75A

Hintergrund: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-?B pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.

UniProt: Q8NAA5