OTUD4 Antikörper (AA 52-1114)

Produktnummer ABIN7875539

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch OTUD4 in WB, ELISA, ICC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für OTUD4 Antikörper (AA 52-1114) (ABIN7875539)

Target: OTUD4 (OTU Domain Containing 4 (OTUD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OTUD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-OTUD4 Antikörper

Bindungsspezifität: AA 52-1114

Verwendungszweck: OTUD4 Antibody / OTU domain-containing protein 4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human OTUD4 recombinant protein (Position: E52-T1114) was used as the immunogen for the OTUD4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the OTUD4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the OTUD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu OTUD4

Target: OTUD4 (OTU Domain Containing 4 (OTUD4))

Andere Bezeichnung: OTUD4

Hintergrund: OTUD4 antibody detects OTU domain-containing protein 4, a deubiquitinating enzyme that regulates protein stability, DNA damage response, and innate immunity. Encoded by the OTUD4 gene on chromosome 4q31.23, this enzyme belongs to the ovarian tumor (OTU) family of cysteine proteases that cleave ubiquitin chains from substrate proteins. OTUD4 maintains cellular protein homeostasis by removing polyubiquitin modifications that target proteins for proteasomal degradation.

Structurally, OTUD4 contains an OTU catalytic domain with conserved cysteine and histidine residues required for thiol protease activity. It also possesses multiple ubiquitin-binding motifs that enable recognition of specific ubiquitin linkages, conferring substrate selectivity. OTUD4 localizes to both cytoplasm and nucleus, where it modulates key signaling proteins involved in stress response, interferon signaling, and genome maintenance.

The OTUD4 antibody is widely used in ubiquitin signaling, oncology, and immunology research to study protein degradation, stability, and DNA repair. Western blot analysis identifies an approximately 130 kilodalton band corresponding to OTUD4, while immunofluorescence reveals both cytoplasmic and nuclear punctate localization, consistent with its regulatory role in ubiquitin metabolism. This antibody enables detection of dynamic changes in ubiquitin-modifying enzymes during stress and immune signaling.

Functionally, OTUD4 stabilizes DNA repair proteins such as KU80 and RPA1, promoting efficient double-strand break repair. It also regulates antiviral responses by modulating the ubiquitination of MAVS and other interferon signaling components, thereby balancing antiviral defense and immune homeostasis. Loss or mutation of OTUD4 impairs genome integrity and contributes to increased sensitivity to genotoxic stress. In cancer, dysregulated OTUD4 expression affects tumor cell proliferation and chemoresistance by altering protein turnover and checkpoint signaling. The OTUD4 antibody provides an essential tool for exploring deubiquitination pathways and their role in cell signaling and genomic maintenance. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring accurate and reproducible detection for ubiquitin and DNA repair studies.

UniProt: Q01804