CBX6 Antikörper (AA 50-236)

Produktnummer ABIN7875333

Der Kaninchen Polyklonal anti-CBX6 Antikörper wird verwendet zum Nachweis von CBX6 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF, ICC und FACS.

Kurzübersicht für CBX6 Antikörper (AA 50-236) (ABIN7875333)

Target: CBX6 (Chromobox Homolog 6 (CBX6))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBX6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-CBX6 Antikörper

Bindungsspezifität: AA 50-236

Verwendungszweck: CBX6 Antibody / Chromobox protein homolog 6

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human CBX6 recombinant protein (Position: D50-K236) was used as the immunogen for the CBX6 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CBX6 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the CBX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu CBX6

Target: CBX6 (Chromobox Homolog 6 (CBX6))

Andere Bezeichnung: CBX6

Hintergrund: CBX6 antibody detects Chromobox protein homolog 6, a member of the chromobox family within the Polycomb group (PcG) proteins, which play a critical role in transcriptional repression and chromatin remodeling. The full UniProt recommended name is Chromobox protein homolog 6 (CBX6), with older or alternate designations including chromobox homolog 6, Polycomb group protein chromobox 6, and human homolog of Drosophila Polycomb protein. CBX6 is a nuclear protein involved in maintaining the repressed state of genes through the formation of polycomb repressive complexes (PRCs), particularly PRC1, which works in conjunction with PRC2 to regulate histone methylation states and gene silencing across development and cell differentiation.

CBX6 binds to trimethylated histone H3 at lysine 27 (H3K27me3), a histone modification mark associated with gene repression, serving as a reader of histone methylation marks and recruiting additional factors to maintain chromatin compaction. It contributes to establishing heritable transcriptional repression, ensuring stable lineage-specific expression patterns in embryonic stem cells and adult tissues. Dysregulation of CBX6 antibody targets, particularly in the Polycomb family, has been associated with aberrant cell proliferation and oncogenic transformation. Overexpression or misexpression of CBX6 has been observed in several cancer types, including hepatocellular carcinoma, prostate cancer, and gliomas, where it may influence epithelial-mesenchymal transition (EMT) and cancer stemness features.

In molecular biology and epigenetic research, CBX6 antibody is widely used for immunofluorescence, chromatin immunoprecipitation (ChIP), and western blot assays to examine Polycomb protein localization and function. It helps distinguish CBX6 from other chromobox homologs (CBX2, CBX4, CBX7, CBX8), each of which has distinct but sometimes overlapping genomic binding profiles and developmental roles. CBX6 has been reported to modulate PRC1 recruitment in concert with histone ubiquitination factors such as RNF2 and BMI1, and to act as a chromatin structural modulator linking histone marks to gene repression. The protein is essential in maintaining pluripotency, regulating HOX gene clusters, and ensuring proper cell fate decisions.

CBX6's gene, CBX6, is located on chromosome 22q13.1. It encodes a protein of approximately 412 amino acids, containing a conserved chromodomain responsible for histone binding and a C-terminal region that mediates protein-protein interactions within the PRC complex. Expression is ubiquitous but higher in testis, placenta, and certain cancerous tissues. Loss-of-function mutations or altered methylation of the CBX6 promoter region can disrupt PRC targeting, influencing gene activation programs and tumor suppressor networks. Studies also show CBX6 modulates long noncoding RNA (lncRNA)-mediated gene silencing, including interactions with lncRNAs such as HOTAIR and MALAT1, further linking CBX6 to chromatin topology and transcriptional memory.

Because of its functional importance, CBX6 serves as a key biomarker in epigenetic and oncogenic signaling studies. NSJ Bioreagents provides validated reagents for detecting CBX6 in tissues across multiple applications including immunohistochemistry, immunoprecipitation, and ChIP-seq.

UniProt: O95503