MADD Antikörper (AA 5-329)

Produktnummer ABIN7875304

Der Kaninchen Polyklonal anti-MADD Antikörper (ABIN7875304) detektiert spezifisch MADD in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MADD Antikörper (AA 5-329) (ABIN7875304)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MADD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-MADD Antikörper

Bindungsspezifität: AA 5-329

Verwendungszweck: MADD Antibody / MAP kinase-activating death domain

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MADD recombinant protein (Position: K5-Y329) was used as the immunogen for the MADD antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MADD antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MADD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Andere Bezeichnung: MADD

Hintergrund: MADD antibody detects MAP kinase-activating death domain protein, encoded by the MADD gene. MAP kinase-activating death domain protein is a multi-domain protein involved in apoptosis regulation, MAPK signaling, and vesicle trafficking. MADD antibody provides researchers with a specific reagent for studying TNF receptor signaling, neurodegeneration, and cancer.

MAP kinase-activating death domain protein interacts with TNF receptor 1 and other death receptors through its death domain, modulating apoptosis and survival. Research using MADD antibody has shown that it can function as an anti-apoptotic factor by inhibiting TNF-induced cell death, while simultaneously promoting MAP kinase signaling pathways that enhance proliferation. This dual role highlights MADD as a regulator of cell fate decisions.

Studies with MADD antibody have revealed that MADD is widely expressed but is particularly abundant in brain, endocrine tissues, and tumors. It is expressed as multiple isoforms through alternative splicing, including long and short forms that differ in their ability to regulate apoptosis and signaling. These isoforms allow MADD to act as a context-dependent modulator of signaling cascades.

Dysregulation of MAP kinase-activating death domain protein has been associated with cancer and neurodegenerative disease. Research using MADD antibody has shown that overexpression supports tumor growth by suppressing apoptosis, while mutations or altered expression in neurons contribute to neurodegenerative pathology. Elevated expression has also been observed in thyroid cancers and other tumors, positioning MADD as a potential biomarker and therapeutic target.

Beyond apoptosis, MADD participates in intracellular trafficking. Research using MADD antibody has demonstrated interactions with Rab3 and related GTPases, regulating synaptic vesicle release and exocytosis. This adds a neurobiological dimension to MADD function and explains its abundance in neurons.

MADD antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting quantifies isoform expression across tissues, immunohistochemistry demonstrates tumor expression, and immunoprecipitation identifies receptor and GTPase interactions. These applications make MADD antibody indispensable for apoptosis, signaling, and cancer research.

By providing validated MADD antibody reagents, NSJ Bioreagents supports studies into TNF receptor pathways, apoptosis, and disease. Detection of MAP kinase-activating death domain protein provides researchers with insight into how death domain proteins regulate survival and pathology.

UniProt: Q8WXG6

Pathways: Caspase Kaskade in der Apoptose