RASAL3 Antikörper (AA 48-925)

Produktnummer ABIN7875219

Der Kaninchen Polyklonal anti-RASAL3 Antikörper wird verwendet zum Nachweis von RASAL3 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ELISA und FACS.

Kurzübersicht für RASAL3 Antikörper (AA 48-925) (ABIN7875219)

Target: RASAL3 (RAS Protein Activator Like 3 (RASAL3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RASAL3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-RASAL3 Antikörper

Bindungsspezifität: AA 48-925

Verwendungszweck: RASAL3 Antibody / Ras GTPase-activating-like protein 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RASAL3 recombinant protein (Position: R48-Q925) was used as the immunogen for the RASAL3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RASAL3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RASAL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RASAL3

Target: RASAL3 (RAS Protein Activator Like 3 (RASAL3))

Andere Bezeichnung: RASAL3

Hintergrund: RASAL3 antibody detects Ras GTPase-activating-like protein 3, a Ras signaling regulator that acts as a negative modulator of Ras activity in immune cells. The UniProt recommended name is Ras GTPase-activating-like protein 3 (RASAL3). This protein converts active GTP-bound Ras into its inactive GDP-bound form, thereby controlling proliferation and activation signals in lymphocytes and other hematopoietic cells.

Functionally, RASAL3 antibody identifies a 944-amino-acid cytoplasmic protein containing C2 and RasGAP domains responsible for lipid membrane binding and GTPase activation. RASAL3 inhibits Ras-dependent pathways, including ERK/MAPK signaling, limiting excessive immune cell activation and promoting homeostasis. It acts downstream of receptor stimulation to fine-tune immune responses.

The RASAL3 gene is located on chromosome 19q13.3 and is highly expressed in natural killer cells, T cells, and macrophages. It serves as a negative feedback regulator of immune activation by controlling Ras signaling intensity following antigen or cytokine stimulation. In NK cells, RASAL3 influences cytotoxic granule release and antiviral responses.

Pathologically, dysregulation of RASAL3 contributes to immune dysfunction and inflammation. Loss of function enhances Ras activity, promoting lymphoproliferative and autoimmune phenotypes. In cancer, RASAL3 downregulation may facilitate tumor immune evasion by disturbing immune regulation. Research using RASAL3 antibody supports investigations into Ras pathway modulation, immune signaling, and oncogenic Ras control.

RASAL3 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect RasGAP proteins and Ras activity regulators. NSJ Bioreagents offers RASAL3 antibody reagents optimized for studies in immune signaling, GTPase biology, and cellular regulation.

Structurally, Ras GTPase-activating-like protein 3 contains an N-terminal C2 lipid-binding domain and a C-terminal catalytic GAP domain. This architecture enables membrane association and inactivation of Ras. This antibody facilitates research on RASAL3's inhibitory role in signal transduction and immune modulation.

UniProt: Q86YV0