HBZ Antikörper (AA 48-142)

Produktnummer ABIN7875179

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HBZ in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Ratte und Maus.

Kurzübersicht für HBZ Antikörper (AA 48-142) (ABIN7875179)

Target: HBZ (Hemoglobin, zeta (HBZ))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HBZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-HBZ Antikörper

Bindungsspezifität: AA 48-142

Verwendungszweck: HBZ Antibody / Hemoglobin zeta

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human HBZ recombinant protein (Position: D48-R142) was used as the immunogen for the HBZ antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HBZ antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HBZ antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu HBZ

Target: HBZ (Hemoglobin, zeta (HBZ))

Andere Bezeichnung: HBZ

Hintergrund: HBZ antibody detects Hemoglobin subunit zeta, encoded by the HBZ gene on chromosome 16p13.3. HBZ antibody is widely used in hematology, developmental biology, and globin gene regulation research. Hemoglobin zeta is an embryonic alpha-like globin expressed during early development in yolk sac-derived erythrocytes. It participates in embryonic hemoglobins (Hb Portland-1 and Hb Portland-2) that provide oxygen transport before fetal hemoglobins are expressed. Expression of HBZ is normally silenced after embryonic development as gamma and beta globins become dominant.

Structurally, Hemoglobin zeta is a ~16 kDa globin protein that forms heterotetramers with epsilon and gamma globins during early development. Its globin fold binds heme, enabling reversible oxygen binding. The protein sequence is highly homologous to alpha globin, reflecting its evolutionary relationship. Despite being developmentally restricted, HBZ can be re-expressed in certain pathologies.

Functionally, Hemoglobin zeta provides oxygen transport in the embryonic circulation and supports survival of primitive erythrocytes. Its transient expression is essential for early development. Researchers use HBZ antibody to investigate globin gene switching, embryonic hematopoiesis, and hemoglobinopathies.

Clinically, persistence of Hemoglobin zeta expression has been observed in some alpha-thalassemias and hematological malignancies. HBZ reactivation may contribute to altered oxygen transport or serve as a biomarker of erythroid stress. Genetic disorders affecting HBZ expression impact early embryonic viability. NSJ Bioreagents provides HBZ antibody for developmental hematology and globin research.

Experimentally, HBZ antibody is used in western blotting to detect the ~16 kDa protein, in immunohistochemistry to study embryonic tissue expression, and in ELISA to quantify hemoglobin zeta in blood samples. Molecular assays with HBZ antibody support studies of globin gene regulation.

UniProt: P02008