APOA1BP Antikörper (AA 46-288)

Produktnummer ABIN7875118

Der Kaninchen Polyklonal anti-APOA1BP Antikörper (ABIN7875118) detektiert spezifisch APOA1BP in WB, IHC, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für APOA1BP Antikörper (AA 46-288) (ABIN7875118)

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APOA1BP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-APOA1BP Antikörper

Bindungsspezifität: AA 46-288

Verwendungszweck: NAXE Antibody / NAD(P)HX epimerase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NAXE recombinant protein (Position: R46-Q288) was used as the immunogen for the NAXE antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NAXE antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NAXE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu APOA1BP

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Andere Bezeichnung: NAXE

Hintergrund: The NAXE antibody targets NAD(P)HX epimerase, an enzyme encoded by the NAXE gene that catalyzes the interconversion of the R and S epimers of NADHX and NADPHX. These damaged forms of NADH and NADPH arise through hydration of nicotinamide cofactors under stress conditions. NAD(P)HX epimerase acts together with NAD(P)HX dehydratase to repair these inactive metabolites, maintaining cofactor homeostasis and protecting cells from metabolic imbalance. The NAXE antibody enables precise detection of this enzyme in studies of cofactor metabolism, mitochondrial function, and cellular stress response.

NAD(P)HX epimerase localizes to both the cytosol and mitochondria, reflecting its role in multiple redox compartments. It is essential for the NAD(P)HX repair system, which safeguards the integrity of cellular redox reactions by regenerating functional NADH and NADPH. The NAXE antibody allows researchers to analyze this protective mechanism, revealing how cells maintain coenzyme pools under conditions of heat, oxidative, or metabolic stress. Loss of NAXE function leads to accumulation of toxic NADHX derivatives, disrupting energy metabolism and enzymatic activity.

Mutations in the NAXE gene cause a rare neurometabolic disorder characterized by recurrent encephalopathy and neurodegeneration, particularly following febrile illness. The NAXE antibody supports mechanistic studies into this disease, helping identify tissue-specific expression and enzyme deficiency in patient-derived samples. Defective NAD(P)HX epimerase activity compromises mitochondrial respiration and redox balance, underscoring its essential role in cellular survival.

Beyond its role in repair metabolism, NAD(P)HX epimerase contributes to stress adaptation and antioxidant defense. It ensures a continuous supply of NAD(P)H required for reductive biosynthesis and detoxification pathways. The NAXE antibody supports quantitative analyses of enzyme regulation in models of oxidative stress, aging, and metabolic disorders. Dysregulation of NAD(P)HX repair enzymes has also been linked to impaired neuronal function and energy deficiency syndromes.

The NAXE antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, providing characteristic mitochondrial and cytosolic staining. NSJ Bioreagents provides this antibody with validated specificity and reproducibility for biochemistry, metabolism, and neuroscience research. By enabling accurate detection of NAD(P)HX epimerase, the NAXE antibody advances understanding of cofactor repair, mitochondrial redox regulation, and neuroprotective metabolic pathways.

UniProt: Q8NCW5