RTL1 Antikörper (AA 446-1327)

Produktnummer ABIN7875046

Der Kaninchen Polyklonal anti-RTL1 Antikörper (ABIN7875046) detektiert spezifisch RTL1 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für RTL1 Antikörper (AA 446-1327) (ABIN7875046)

Target: RTL1 (Retrotransposon-Like 1 (RTL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RTL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-RTL1 Antikörper

Bindungsspezifität: AA 446-1327

Verwendungszweck: RTL1 Antibody / Retrotransposon like 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RTL1 recombinant protein (Position: H446-R1327) was used as the immunogen for the RTL1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RTL1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RTL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RTL1

Target: RTL1 (Retrotransposon-Like 1 (RTL1))

Andere Bezeichnung: RTL1

Hintergrund: RTL1 antibody detects Retrotransposon-derived protein 1, encoded by the RTL1 gene on chromosome 14q32. RTL1 antibody is commonly used in research on placental biology, imprinting, and developmental regulation. RTL1, also known as PEG11 (paternally expressed gene 11), is an imprinted gene derived from a retrotransposon and plays essential roles in placental development, particularly in maintaining fetal capillary endothelial cells within the labyrinth layer of the placenta.

Structurally, RTL1 is a ~155 kDa protein with retroviral Gag and Pol-like domains, reflecting its origin from an ancient retrotransposon. Although lacking enzymatic retroviral activity, RTL1 retains structural features that support interactions with cellular machinery. Its expression is tightly regulated by imprinting, with only the paternal allele being active. RTL1 expression is restricted to placenta and certain fetal tissues, consistent with its developmental functions.

Functionally, RTL1 supports placental vascular development and fetal-maternal exchange. Knockout studies in mice reveal mid-gestation lethality due to defective placental capillaries. Overexpression, conversely, disrupts placental balance and fetal growth. RTL1 expression is regulated by antisense microRNAs encoded in the imprinted cluster, ensuring precise dosage. Researchers use RTL1 antibody to investigate placental biology, imprinting disorders, and retroelement-derived gene functions.

Clinically, altered RTL1 expression is associated with human imprinting disorders such as Kagami-Ogata syndrome and Temple syndrome, which involve abnormal dosage from the 14q32 region. Dysregulation of RTL1 may contribute to abnormal fetal growth and placental insufficiency. Its retrotransposon-derived nature highlights how ancient viral elements have been co-opted into mammalian development. NSJ Bioreagents provides RTL1 antibody for use in developmental biology and imprinting research.

Experimentally, RTL1 antibody is used in western blotting to detect the ~155 kDa protein, in immunohistochemistry to examine placental tissue, and in RNA-protein studies to assess regulatory interactions. It is a valuable tool for investigating imprinted gene expression and placental function.

UniProt: A6NKG5