Involucrin Antikörper (AA 421-568)

Produktnummer ABIN7874933

Dieser Maus Monoklonal Antikörper detektiert spezifisch Involucrin in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Involucrin Antikörper (AA 421-568) (ABIN7874933)

Target: Involucrin (IVL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM259

Produktdetails anti-Involucrin Antikörper

Bindungsspezifität: AA 421-568

Verwendungszweck: Involucrin Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: Involucrin

Hintergrund: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

UniProt: P07476