SPERT Antikörper (AA 40-448)

Produktnummer ABIN7874700

Der Kaninchen Polyklonal anti-SPERT Antikörper wird verwendet zum Nachweis von SPERT in Proben von Human, Ratte und Maus. Er wurde validiert für ELISA, WB und FACS.

Kurzübersicht für SPERT Antikörper (AA 40-448) (ABIN7874700)

Target: SPERT (Spermatid Associated (SPERT))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPERT Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-SPERT Antikörper

Bindungsspezifität: AA 40-448

Verwendungszweck: CBY2 Antibody / Chibby 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human CBY2 recombinant protein (Position: E40-V448) was used as the immunogen for the CBY2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CBY2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the CBY2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SPERT

Target: SPERT (Spermatid Associated (SPERT))

Andere Bezeichnung: CBY2

Hintergrund: CBY2 antibody detects Chibby homolog 2, a centrosome- and cilium-associated protein involved in ciliogenesis, cell polarity, and Wnt signaling regulation. Encoded by the CBY2 gene on chromosome 12q24.13, this protein belongs to the Chibby family, which includes CBY1 and CBY3, known for modulating beta-catenin signaling and ciliary structure. CBY2 contains coiled-coil domains that mediate dimerization and interaction with other ciliary proteins such as CEP164 and ODF2. It localizes to the basal body and transition zone of cilia, where it contributes to the assembly and stabilization of the ciliary axoneme.

CBY2 is expressed in ciliated tissues including respiratory epithelium, reproductive organs, and brain ependyma. It plays an essential role in the early stages of ciliogenesis by promoting docking of basal bodies to the plasma membrane and initiating axoneme extension. Disruption of CBY2 function leads to defective ciliogenesis, altered epithelial polarity, and impaired signaling through cilia-dependent pathways such as Hedgehog and Wnt. In reproductive cells, CBY2 contributes to flagellar formation and sperm motility, while in neurons it supports ependymal cilia function crucial for cerebrospinal fluid flow.

The CBY2 antibody is widely used in cell biology and developmental studies to analyze ciliary formation, centrosomal organization, and beta-catenin signaling. Western blot analysis detects a 42 kilodalton band corresponding to full-length CBY2, and immunofluorescence reveals punctate staining at basal bodies marked by gamma-tubulin. In cilia-deficient models, CBY2 expression serves as a marker for disrupted ciliogenesis. The antibody is also useful for exploring CBY2's role in planar cell polarity and coordination of multiciliated cell differentiation.

Mutations or reduced expression of CBY2 have been linked to ciliopathies, infertility, and hydrocephalus, reflecting its vital role in ciliary assembly. The CBY2 antibody provides a powerful tool for investigating these disorders and for mapping ciliary protein networks in epithelial and germline systems. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reliable results in research on centrosome and cilia biology.

UniProt: Q8NA61