GK5 Antikörper (AA 37-402)

Produktnummer ABIN7874403

Der Kaninchen Polyklonal anti-GK5 Antikörper wird verwendet zum Nachweis von GK5 in Proben von Human. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für GK5 Antikörper (AA 37-402) (ABIN7874403)

Target: GK5 (Glycerol Kinase 5 (Putative) (GK5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GK5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-GK5 Antikörper

Bindungsspezifität: AA 37-402

Verwendungszweck: GK5 Antibody / Glycerol kinase 5

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human GK5 recombinant protein (Position: D37-K402) was used as the immunogen for the GK5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GK5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GK5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GK5

Target: GK5 (Glycerol Kinase 5 (Putative) (GK5))

Andere Bezeichnung: GK5

Hintergrund: The GK5 antibody targets Glycerol kinase 5, a mitochondrial and peroxisomal enzyme encoded by the GK5 gene. Glycerol kinase 5 phosphorylates glycerol to glycerol-3-phosphate, a key intermediate in lipid metabolism and energy homeostasis. As a member of the glycerol kinase family, this enzyme contributes to triglyceride synthesis, fatty-acid metabolism, and membrane lipid turnover. The GK5 antibody provides a reliable reagent for studying glycerol utilization, lipid signaling, and mitochondrial metabolic integration.

Glycerol kinase 5 is distinct from the canonical cytosolic glycerol kinase (GK1) in its subcellular localization and tissue specificity. It is highly expressed in testis and skin, where it may regulate lipid composition during spermatogenesis and barrier formation. The GK5 antibody allows detection of this enzyme in mitochondria-rich tissues, helping define its contribution to energy production and lipid biosynthesis. By phosphorylating glycerol, GK5 supports the glycerol phosphate shuttle and links carbohydrate metabolism with lipid anabolism.

Structurally, Glycerol kinase 5 shares conserved ATP-binding and catalytic residues with other glycerol kinases but contains mitochondrial targeting sequences. It associates with mitochondrial membranes, where it modulates local glycerol metabolism. The GK5 antibody supports subcellular localization studies that examine mitochondrial lipid synthesis and oxidative phosphorylation coupling. In skin and reproductive tissues, GK5 may play roles in lipid barrier maintenance and sperm energy metabolism.

Altered expression of Glycerol kinase 5 has been observed in metabolic disorders and certain cancers. Its involvement in lipid signaling suggests that GK5 activity could affect membrane dynamics and cellular energy balance. The GK5 antibody facilitates quantification of expression changes under metabolic stress, nutrient deprivation, or differentiation. Functional studies indicate that GK5 may participate in adaptive metabolic responses that sustain energy production under shifting substrate conditions.

The GK5 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing punctate mitochondrial staining consistent with its predicted localization. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for lipid metabolism and mitochondrial research. By enabling precise detection of Glycerol kinase 5, the GK5 antibody supports studies into glycerol utilization, energy regulation, and metabolic cross-talk between lipid and carbohydrate pathways.

UniProt: Q6ZS86