RLIM Antikörper (AA 369-621)

Produktnummer ABIN7874379

Der Kaninchen Polyklonal anti-RLIM Antikörper wird verwendet zum Nachweis von RLIM in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC, IF und FACS.

Kurzübersicht für RLIM Antikörper (AA 369-621) (ABIN7874379)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RLIM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-RLIM Antikörper

Bindungsspezifität: AA 369-621

Verwendungszweck: RLIM Antibody / RING finger protein LIM domain-interacting

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RLIM recombinant protein (Position: R369-E621) was used as the immunogen for the RLIM antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RLIM antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RLIM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Andere Bezeichnung: RLIM

Hintergrund: RLIM antibody detects RING finger protein LIM domain-interacting (RLIM), also known as RNF12, encoded by the RLIM gene on chromosome Xq13.2. RLIM antibody is commonly used in studies of ubiquitination, X-chromosome inactivation, and transcriptional regulation. RLIM is an E3 ubiquitin ligase that regulates protein stability, signaling, and developmental processes. It is expressed broadly, with high levels in embryonic tissues and reproductive organs, and is essential for early embryogenesis and sex-specific gene regulation.

Structurally, RLIM is a ~600 amino acid protein containing a C-terminal RING finger domain responsible for ubiquitin ligase activity and N-terminal regions that mediate protein-protein interactions. It interacts with LIM domain transcription factors, modulating their activity and stability. Alternative splicing generates multiple isoforms with differences in subcellular localization and activity. Nuclear and cytoplasmic pools of RLIM allow it to function in both transcriptional regulation and cytoplasmic protein turnover.

Functionally, RLIM acts as an E3 ubiquitin ligase, tagging proteins for proteasomal degradation. It regulates LIM homeodomain transcription factors by modulating their stability, thereby controlling gene expression programs during development. RLIM also plays a crucial role in X-chromosome inactivation by promoting degradation of the transcription factor REX1, enabling proper expression of Xist RNA. Additional roles include regulation of BMP and TGF-b signaling pathways, neuronal differentiation, and germ cell development. Researchers use RLIM antibody to study ubiquitination, transcriptional regulation, and embryonic development.

Clinically, RLIM mutations are associated with intellectual disability, developmental delay, and congenital anomalies, reflecting its importance in brain and body development. As an X-linked gene, mutations often present with sex-specific patterns of inheritance. RLIM dysfunction has been implicated in cancer, where altered ubiquitination affects cell growth and signaling. NSJ Bioreagents provides RLIM antibody for developmental biology, cancer, and molecular signaling research.

Experimentally, RLIM antibody is used in western blotting to detect the ~68 kDa protein, in immunofluorescence to study nuclear and cytoplasmic localization, and in immunohistochemistry to examine expression in embryonic and tumor tissues. Co-immunoprecipitation with RLIM antibody identifies interacting transcription factors and ubiquitination substrates.

UniProt: Q9NVW2