GLT25D1 Antikörper (AA 34-454)

Produktnummer ABIN7874081

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GLT25D1 in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für GLT25D1 Antikörper (AA 34-454) (ABIN7874081)

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLT25D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-GLT25D1 Antikörper

Bindungsspezifität: AA 34-454

Verwendungszweck: COLGALT1 Antibody / Collagen beta(1-O)galactosyltransferase 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454) was used as the immunogen for the COLGALT1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the COLGALT1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the COLGALT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GLT25D1

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Andere Bezeichnung: COLGALT1

Hintergrund: COLGALT1 antibody detects Collagen beta(1-O)galactosyltransferase 1, an enzyme that catalyzes the transfer of galactose to hydroxylysine residues in collagen. This post-translational modification is essential for proper collagen folding, stability, and secretion. COLGALT1 functions within the endoplasmic reticulum and Golgi apparatus and contributes to the biosynthesis of structurally mature collagen fibers. The COLGALT1 antibody is widely used in connective tissue, developmental, and fibrosis research to study collagen maturation, glycosylation, and extracellular matrix regulation.

COLGALT1 is encoded by the COLGALT1 gene located on human chromosome 19p13.2. The protein is approximately 739 amino acids long and is a member of the galactosyltransferase family. It forms a functional complex with its homolog COLGALT2 and works sequentially with lysyl hydroxylase (PLOD family) enzymes to glycosylate hydroxylysine residues in collagen alpha chains. This process stabilizes triple helix formation and enhances collagen secretion and cross-linking.

The COLGALT1 antibody detects an 85 kilodalton protein by western blot and shows perinuclear staining under immunofluorescence microscopy consistent with Golgi localization. COLGALT1 activity is required for the synthesis of type I, II, III, and IV collagens. Deficiency or mutation results in defective collagen glycosylation, leading to abnormal extracellular matrix assembly and diseases such as Ehlers-Danlos syndrome and osteogenesis imperfecta-like disorders.

COLGALT1 expression is tightly regulated during development and tissue repair. Increased expression promotes collagen deposition in fibrotic tissues, whereas reduced expression leads to unstable collagen and tissue fragility. It also contributes to tumor stroma remodeling by enhancing extracellular matrix density and rigidity, influencing cancer cell migration and angiogenesis.

As a key enzyme linking collagen biosynthesis and tissue integrity, COLGALT1 serves as a critical target for studying connective tissue disorders and fibrotic diseases. NSJ Bioreagents provides a validated COLGALT1 antibody optimized for its applications, supporting research into collagen glycosylation, fibrosis, and tissue regeneration.

UniProt: Q8NBJ5

Pathways: SARS-CoV-2 Protein Interaktom