HIBADH Antikörper (AA 34-332)

Produktnummer ABIN7874076

Der Kaninchen Polyklonal anti-HIBADH Antikörper wird verwendet zum Nachweis von HIBADH in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für HIBADH Antikörper (AA 34-332) (ABIN7874076)

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIBADH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-HIBADH Antikörper

Bindungsspezifität: AA 34-332

Verwendungszweck: HIBADH Antibody / 3-Hydroxyisobutyrate dehydrogenase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human HIBADH recombinant protein (Position: R34-E332) was used as the immunogen for the HIBADH antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HIBADH antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HIBADH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu HIBADH

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Andere Bezeichnung: HIBADH

Hintergrund: HIBADH antibody detects 3-hydroxyisobutyrate dehydrogenase, a mitochondrial enzyme that catalyzes a critical step in valine catabolism. Encoded by the HIBADH gene on chromosome 7q31.1, this enzyme converts 3-hydroxyisobutyrate to methylmalonate semialdehyde using NAD+ as a cofactor. HIBADH plays an important role in branched-chain amino acid degradation and energy production by linking valine metabolism to the tricarboxylic acid (TCA) cycle. It is primarily expressed in liver, kidney, muscle, and other energy-demanding tissues.

HIBADH localizes to the mitochondrial matrix, where it functions within the valine degradation pathway that ultimately generates succinyl-CoA for entry into the TCA cycle. This enzymatic step contributes to both energy generation and metabolic flexibility, especially during fasting or muscle exertion. In addition to its metabolic role, HIBADH helps regulate mitochondrial redox balance through NAD+/NADH interconversion. Mutations or deficiencies in this enzyme may disrupt amino acid catabolism and contribute to metabolic disorders involving organic acid accumulation.

The HIBADH antibody is used in metabolism, mitochondrial, and enzymology research to detect mitochondrial dehydrogenases and characterize amino acid catabolic pathways. Western blot analysis identifies a 35 kilodalton band corresponding to HIBADH, while immunofluorescence reveals punctate mitochondrial staining. This antibody provides a valuable reagent for studying mitochondrial metabolism, oxidative phosphorylation, and amino acid utilization under physiological and disease conditions.

Beyond energy metabolism, altered HIBADH expression has been linked to mitochondrial dysfunction, muscle wasting, and cancer metabolism. Its regulation reflects cellular metabolic adaptation to nutrient and energy demands. The HIBADH antibody is validated by NSJ Bioreagents for western blotting and flow cytometry, providing consistent performance for the analysis of mitochondrial enzymatic function and metabolic flux regulation.

UniProt: P31937