HTRA4 Antikörper (AA 31-464)

Produktnummer ABIN7873789

Der Kaninchen Polyklonal anti-HTRA4 Antikörper (ABIN7873789) detektiert spezifisch HTRA4 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HTRA4 Antikörper (AA 31-464) (ABIN7873789)

Target: HTRA4 (HtrA Serine Peptidase 4 (HTRA4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HTRA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-HTRA4 Antikörper

Bindungsspezifität: AA 31-464

Verwendungszweck: HTRA4 Antibody / Serine protease HTRA4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human HTRA4 recombinant protein (Position: A31-D464) was used as the immunogen for the HTRA4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HTRA4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HTRA4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu HTRA4

Target: HTRA4 (HtrA Serine Peptidase 4 (HTRA4))

Andere Bezeichnung: HTRA4

Hintergrund: HTRA4 antibody detects Serine protease HTRA4, a member of the high-temperature requirement A (HTRA) family of serine proteases involved in protein quality control, stress response, and apoptosis. The UniProt recommended name is Serine protease HTRA4 (HTRA4). This enzyme participates in proteolytic degradation of misfolded or damaged proteins and contributes to cellular stress adaptation, particularly in placental and cardiovascular tissues.

Functionally, HTRA4 antibody identifies a 476-amino-acid secreted and intracellular protease that possesses both trypsin-like catalytic activity and PDZ domain-mediated substrate recognition. HTRA4 cleaves misfolded or aggregated proteins to maintain proteostasis and prevent cellular toxicity. It is expressed primarily in the placenta, where it regulates trophoblast differentiation and contributes to placental remodeling. Additionally, HTRA4 can influence mitochondrial homeostasis and apoptosis by degrading components of the mitochondrial protein import machinery during stress conditions.

The HTRA4 gene is located on chromosome 8p13.3 and is highly expressed in placental trophoblasts, heart, and lung. Its expression increases during pregnancy and hypoxia, reflecting roles in stress response and tissue remodeling. Like other HTRA proteases, HTRA4 combines chaperone-like and proteolytic activities to ensure proper protein folding and clearance.

Pathologically, elevated HTRA4 levels have been linked to preeclampsia, where excessive proteolytic activity contributes to endothelial dysfunction and vascular injury. Dysregulation may also play a role in cardiac remodeling, pulmonary hypertension, and neurodegenerative disease by altering extracellular matrix integrity and stress signaling. Research using HTRA4 antibody supports studies in placental biology, protease function, and oxidative stress response.

HTRA4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect serine proteases involved in protein homeostasis. NSJ Bioreagents provides HTRA4 antibody reagents optimized for studies in stress regulation, apoptosis, and proteostasis pathways.

Structurally, Serine protease HTRA4 contains an N-terminal signal peptide, a trypsin-like catalytic domain with the conserved serine-histidine-aspartate triad, and a C-terminal PDZ domain that mediates substrate recognition and oligomerization. These structural features enable substrate-specific proteolysis and regulated activation under stress. This antibody facilitates analysis of HTRA4's function in proteostasis, placental health, and cardiovascular pathology.

UniProt: P83105