PARG Antikörper (AA 27-854)

Produktnummer ABIN7873146

Der Kaninchen Polyklonal anti-PARG Antikörper wird verwendet zum Nachweis von PARG in Proben von Human. Er wurde validiert für WB, ELISA, ICC, IF und FACS.

Kurzübersicht für PARG Antikörper (AA 27-854) (ABIN7873146)

Target: PARG

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-PARG Antikörper

Bindungsspezifität: AA 27-854

Verwendungszweck: PARG Antibody / Poly(ADP-ribose) glycohydrolase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PARG recombinant protein (Position: D27-R854) was used as the immunogen for the PARG antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PARG antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PARG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PARG

Target: PARG

Andere Bezeichnung: PARG

Hintergrund: PARG antibody detects Poly(ADP-ribose) glycohydrolase, encoded by the PARG gene on chromosome 10q11.23. PARG antibody is widely used in DNA damage response, chromatin remodeling, and cancer biology research. PARG is the main enzyme responsible for degrading poly(ADP-ribose) chains, which are synthesized by PARP family enzymes in response to DNA breaks and stress signals. By hydrolyzing poly(ADP-ribose), PARG resets chromatin state and restores protein activity following PARP-mediated modifications.

Structurally, PARG is a ~110 kDa enzyme with a macrodomain-containing catalytic region. It cleaves glycosidic ribose-ribose bonds in poly(ADP-ribose) chains, generating free ADP-ribose units. PARG localizes predominantly to the nucleus but is also found in cytoplasm and mitochondria, reflecting diverse roles in cellular stress responses. Alternative isoforms, including shorter forms, may localize differently and regulate specific processes.

Functionally, PARG counteracts PARP activity to maintain balance in ADP-ribosylation signaling. After DNA damage, PARP1 rapidly polymerizes ADP-ribose chains that recruit DNA repair factors. PARG then degrades these chains to terminate signaling, recycle NAD+, and permit chromatin resetting. Dysregulation of PARG disrupts repair efficiency and genomic stability. Researchers use PARG antibody to study DNA repair, chromatin biology, and PARP inhibitor responses.

Clinically, PARG is relevant in cancer therapy because PARP inhibitors exploit defective homologous recombination repair in tumors. PARG inhibition further sensitizes cancer cells to DNA damage, making it a therapeutic target. PARG dysregulation has also been implicated in neurodegeneration, where abnormal ADP-ribose metabolism contributes to cell death. NSJ Bioreagents supplies PARG antibody for research in DNA repair, cancer biology, and therapeutic development.

Experimentally, PARG antibody is applied in western blotting to detect the ~110 kDa protein, in immunohistochemistry to study nuclear expression, and in enzymatic assays to monitor ADP-ribose metabolism. Co-immunoprecipitation with PARG antibody identifies complexes with PARP and DNA repair proteins.

UniProt: Q86W56