FOXJ1 Antikörper (AA 27-405)

Produktnummer ABIN7873119

Der Kaninchen Polyklonal anti-FOXJ1 Antikörper wird verwendet zum Nachweis von FOXJ1 in Proben von Human und Ratte. Er wurde validiert für WB, ELISA, FACS, IF und IHC (p).

Kurzübersicht für FOXJ1 Antikörper (AA 27-405) (ABIN7873119)

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXJ1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXJ1 Antikörper

Bindungsspezifität: AA 27-405

Verwendungszweck: HFH-4 Antibody / FOXJ1

Aufreinigung: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids D27-A405) was used as the immunogen for the HFH-4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HFH-4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HFH-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FOXJ1

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Andere Bezeichnung: HFH-4

Hintergrund: HFH-4 antibody targets Forkhead box protein J1, also known as FOXJ1, a nuclear transcription factor that serves as a master regulator of motile ciliogenesis. HFH-4 was the original name assigned to this protein before standardized forkhead box nomenclature was adopted, and it remains widely used in the literature. FOXJ1 is a member of the forkhead box family of transcription factors and is predominantly localized to the nucleus, where it regulates gene expression programs required for the formation and function of motile cilia.

HFH-4 antibody is commonly used to study ciliated epithelial cells in tissues where coordinated ciliary movement is essential for normal physiology. FOXJ1 expression is especially prominent in the respiratory epithelium, ependymal cells lining the brain ventricles, reproductive tract epithelia, and other specialized tissues containing motile cilia. Its expression marks cells committed to a motile ciliated lineage, distinguishing them from cells bearing primary, non-motile cilia.

Functionally, FOXJ1 drives the transcription of genes involved in basal body docking, axoneme assembly, and ciliary motility. By activating these downstream targets, HFH-4 ensures proper development of multiciliated cells and coordinated ciliary beating. Loss or disruption of FOXJ1 expression results in defective ciliogenesis and impaired fluid movement, underscoring its essential role in tissue homeostasis and developmental processes.

HFH-4 antibody is also valuable in disease-related research. Abnormal FOXJ1 expression or function has been associated with primary ciliary dyskinesia, hydrocephalus, chronic respiratory disease, and infertility, all of which arise from dysfunctional motile cilia. In the central nervous system, FOXJ1-positive ependymal cells are critical for cerebrospinal fluid flow, and altered expression has been studied in neurodevelopmental disorders and injury responses.

In cancer research, HFH-4 antibody is used as a lineage and differentiation marker in certain tumors, particularly those with ciliated or ependymal characteristics. FOXJ1 expression can provide insight into tumor origin, cellular differentiation state, and transcriptional regulation in both normal and pathological contexts. Its relatively restricted expression pattern enhances its utility as a specific marker in histological and molecular studies.

At the genomic level, the FOXJ1 gene is located on human chromosome 17 and encodes a protein containing a conserved forkhead DNA-binding domain. This domain enables sequence-specific transcriptional regulation of ciliogenesis-related genes. HFH-4 antibody supports research into these regulatory networks by enabling detection of FOXJ1 expression across tissues, developmental stages, and experimental models relevant to cilia biology and epithelial differentiation.

UniProt: Q92949

Pathways: Regulation of Leukocyte Mediated Immunity