IFI30 Antikörper (AA 27-250)

Produktnummer ABIN7873105

Der Kaninchen Polyklonal anti-IFI30 Antikörper (ABIN7873105) detektiert spezifisch IFI30 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für IFI30 Antikörper (AA 27-250) (ABIN7873105)

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFI30 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-IFI30 Antikörper

Bindungsspezifität: AA 27-250

Verwendungszweck: IFI30 Antibody / GILT / Gamma-interferon-inducible lysosomal thiol reductase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human IFI30 recombinant protein (Position: S27-K250) was used as the immunogen for the IFI30 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the IFI30 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the IFI30 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu IFI30

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Andere Bezeichnung: IFI30

Hintergrund: IFI30 antibody detects Gamma-interferon-inducible lysosomal thiol reductase, an enzyme that catalyzes the reduction of disulfide bonds in endosomal and lysosomal compartments. The UniProt recommended name is Gamma-interferon-inducible lysosomal thiol reductase (IFI30). This enzyme facilitates antigen processing by reducing disulfide-linked epitopes within endosomes, promoting peptide loading onto MHC class II molecules for immune presentation.

Functionally, IFI30 antibody identifies a 250-amino-acid glycoprotein localized to lysosomes and late endosomes. IFI30 acts in concert with gamma-interferon-induced pathways to maintain redox balance within antigen-presenting cells. Its active site cysteines participate in thiol-disulfide exchange reactions, regenerating reduced peptides that can be efficiently bound by MHC class II for recognition by CD4+ T cells.

The IFI30 gene is located on chromosome 19p13.1 and is expressed predominantly in macrophages, dendritic cells, and B lymphocytes. Its expression is upregulated by interferon-gamma signaling and inflammatory cytokines. IFI30 functions downstream of antigen uptake and proteolysis, serving as a critical component in adaptive immune activation and tolerance maintenance.

Pathologically, altered IFI30 activity has been linked to immune disorders and cancer. Overexpression can enhance antigen presentation and immune surveillance, while deficiencies impair immune responses and tolerance mechanisms. IFI30 is also explored as a marker of macrophage activation and tumor immunogenicity. Research using IFI30 antibody supports immunology, antigen processing, and redox regulation studies.

IFI30 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal enzymes and immune regulators. NSJ Bioreagents provides validated IFI30 antibody reagents for use in immunology, cell biology, and redox regulation research.

Structurally, Gamma-interferon-inducible lysosomal thiol reductase contains a thioredoxin-like domain and a signal peptide targeting it to lysosomes. The protein undergoes proteolytic maturation to its active form within acidic compartments. This antibody facilitates the study of IFI30's enzymatic and immunomodulatory roles in antigen processing and cellular redox maintenance.

UniProt: P13284