MED4 Antikörper (AA 27-247)

Produktnummer ABIN7873104

Der Kaninchen Polyklonal anti-MED4 Antikörper wird verwendet zum Nachweis von MED4 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, ELISA, IHC, IF, ICC und FACS.

Kurzübersicht für MED4 Antikörper (AA 27-247) (ABIN7873104)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-MED4 Antikörper

Bindungsspezifität: AA 27-247

Verwendungszweck: MED4 Antibody / Mediator complex subunit 4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MED4 recombinant protein (Position: R27-H247) was used as the immunogen for the MED4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MED4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MED4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Andere Bezeichnung: MED4

Hintergrund: MED4 antibody recognizes Mediator complex subunit 4, a nuclear protein encoded by the MED4 gene located on chromosome 13q12.2. MED4 is a component of the multiprotein Mediator complex, which serves as an essential coactivator linking transcription factors to RNA polymerase II during gene transcription. The Mediator complex regulates nearly all RNA polymerase II-dependent genes, integrating signals from diverse transcriptional activators and repressors to modulate gene expression. Structurally, MED4 is a small subunit localized in the head module of the Mediator complex, where it contributes to the stabilization of interactions between the Mediator and general transcription factors such as TFIIB and TFIIH.

MED4 antibody detects a protein that resides in the nucleus and is widely expressed across tissues, with particularly high expression in actively proliferating cells. MED4 functions as a structural component that helps assemble and maintain the integrity of the Mediator complex. The protein interacts with other Mediator subunits, including MED6, MED7, and MED8, to promote preinitiation complex formation and transcriptional activation. Through its participation in this multi-subunit assembly, MED4 indirectly influences processes such as cell cycle regulation, metabolic control, and differentiation.

Functionally, MED4 acts as a coregulator for a variety of signaling pathways, including those mediated by nuclear hormone receptors, Myc, and p53. The Mediator complex serves as a bridge between enhancer-bound transcription factors and the basal transcriptional machinery, making MED4 indispensable for transcriptional initiation and elongation. Disruption of MED4 or other Mediator components can impair RNA synthesis and contribute to developmental defects or disease. Recent studies suggest that the Mediator complex, including MED4, may participate in transcriptional pausing and gene-specific regulation in response to environmental and stress cues.

Mutations or copy number variations affecting the MED4 gene have been implicated in neurodevelopmental disorders and congenital anomalies, underscoring its role in transcriptional control during embryogenesis. Aberrant expression of MED4 has also been observed in various cancers, where altered Mediator activity promotes oncogenic transcriptional programs. Functional proteomics analyses show that MED4 participates in chromatin looping and enhancer-promoter communication, critical for high-fidelity transcriptional activation. Structurally, MED4's helical regions allow it to form coiled-coil interactions within the Mediator head domain, ensuring complex stability.

In disease contexts, dysregulated Mediator signaling has been associated with metabolic syndromes, inflammatory diseases, and tumor progression. MED4 expression correlates with proliferative gene signatures in certain cancers, suggesting its potential role as a biomarker of active transcriptional states. Because of its conserved function, MED4 serves as an essential reference subunit for Mediator complex studies across species.

Immunohistochemical staining using MED4 antibody demonstrates nuclear localization in epithelial and neuronal tissues, consistent with its function in transcriptional regulation. MED4 antibody from NSJ Bioreagents supports research in transcriptional biology, gene regulation, and the molecular mechanisms underlying human disease linked to Mediator complex dysfunction.

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha