SH2B1 Antikörper (AA 26-715)

Produktnummer ABIN7873014

Der Kaninchen Polyklonal anti-SH2B1 Antikörper wird verwendet zum Nachweis von SH2B1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für SH2B1 Antikörper (AA 26-715) (ABIN7873014)

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SH2B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-SH2B1 Antikörper

Bindungsspezifität: AA 26-715

Verwendungszweck: SH2B1 Antibody / SH2B adapter protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SH2B1 recombinant protein (Position: W26-Q715) was used as the immunogen for the SH2B1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SH2B1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SH2B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SH2B1

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Andere Bezeichnung: SH2B1

Hintergrund: SH2B1 antibody detects SH2B adapter protein 1, a signaling adaptor that regulates insulin, leptin, and growth factor receptor pathways controlling metabolism and energy balance. Encoded by the SH2B1 gene on chromosome 16p11.2, this cytoplasmic adaptor belongs to the SH2B family of signal transduction proteins and serves as a key modulator of receptor tyrosine kinase and cytokine receptor signaling. SH2B1 enhances insulin receptor signaling, JAK2 activation, and downstream PI3K and MAPK pathways involved in glucose metabolism, cell growth, and neuronal signaling.

Structurally, SH2B1 is a 756-amino-acid protein of approximately 85 kilodaltons containing an N-terminal dimerization domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain that mediates phosphotyrosine-dependent interactions. It exists in multiple isoforms (alpha, beta, gamma, and delta) produced by alternative splicing, with tissue-specific expression across brain, liver, adipose tissue, and skeletal muscle. The protein acts as a scaffold that bridges receptor kinases to intracellular effectors, amplifying or modulating signaling intensity and duration.

The SH2B1 antibody is widely used in metabolism, endocrinology, and neurobiology research to study insulin signaling, leptin sensitivity, and receptor-mediated pathways regulating energy homeostasis. Western blot analysis detects a band of approximately 85 kilodaltons corresponding to SH2B1, while immunofluorescence reveals cytoplasmic and plasma membrane localization. This antibody is an essential reagent for exploring the molecular basis of metabolic signaling and neuronal regulation.

Functionally, SH2B1 binds to insulin and leptin receptors through JAK2 and IRS1, enhancing phosphorylation cascades that regulate glucose uptake and appetite control. Loss or mutation of SH2B1 disrupts these pathways, leading to insulin resistance, obesity, and metabolic syndrome. Deletions encompassing SH2B1 are associated with severe early-onset obesity and neurobehavioral abnormalities, while overexpression can increase leptin sensitivity and improve glucose tolerance. Beyond metabolism, SH2B1 contributes to neuronal growth, regeneration, and synaptic plasticity through its modulation of NGF and BDNF signaling. The SH2B1 antibody provides a robust tool for studying these signaling mechanisms and their implications in metabolic and neurological disorders. NSJ Bioreagents validates this antibody for its applications, ensuring specific and reproducible results across biological systems.

UniProt: Q9NRF2