NAB2 Antikörper (AA 235-525)

Produktnummer ABIN7872620

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NAB2 in WB, ELISA, ICC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NAB2 Antikörper (AA 235-525) (ABIN7872620)

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-NAB2 Antikörper

Bindungsspezifität: AA 235-525

Verwendungszweck: NAB2 Antibody / NGFI-A-binding protein 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NAB2 recombinant protein (Position: D235-Q525) was used as the immunogen for the NAB2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NAB2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NAB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NAB2

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Andere Bezeichnung: NAB2

Hintergrund: The NAB2 antibody targets NGFI-A-binding protein 2, a transcriptional corepressor encoded by the NAB2 gene. This protein modulates gene expression by interacting with EGR (early growth response) family transcription factors, particularly EGR1 and EGR2, to regulate cell differentiation, proliferation, and nerve development. NGFI-A-binding protein 2 acts as both a transcriptional repressor and activator depending on context, influencing neuronal signaling, myelination, and growth factor response pathways. The NAB2 antibody enables specific detection of this multifunctional nuclear protein in studies of gene regulation and neurodevelopment.

NGFI-A-binding protein 2 contains two conserved EGR-binding domains and a repression domain that interacts with the chromatin remodeling machinery. Through these domains, NAB2 forms complexes with EGR transcription factors to fine-tune expression of immediate-early genes involved in cellular differentiation. The NAB2 antibody allows visualization of these interactions in neural and non-neural tissues, facilitating studies on transcriptional control mechanisms that balance growth and differentiation. In the nervous system, NAB2 is essential for Schwann cell maturation and myelin formation, acting downstream of EGR2 signaling.

Mutations or dysregulation of NGFI-A-binding protein 2 have been linked to neuropathies and developmental disorders. NAB2 deficiency in animal models disrupts myelination and peripheral nerve function, underscoring its importance in glial differentiation. The NAB2 antibody supports investigations into these phenotypes by enabling detection of expression patterns during neural development and regeneration. It also serves as a valuable tool for examining NAB2's role in non-neuronal contexts, including cardiac hypertrophy, vascular remodeling, and hormonal signaling.

NAB2 has gained attention in oncology due to the formation of NAB2-STAT6 gene fusions, which drive solitary fibrous tumors. The fusion protein combines the NAB2 repression domain with the STAT6 activation domain, leading to constitutive transcriptional activation. The NAB2 antibody aids in identifying expression changes associated with these fusion events, supporting tumor classification and mechanistic studies. NAB2's broader roles in transcriptional regulation and growth control make it a versatile subject for molecular and pathological research.

The NAB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding strong nuclear staining consistent with its transcriptional function. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent suitable for cell biology, neuroscience, and cancer research. By enabling detailed analysis of NGFI-A-binding protein 2 expression and localization, the NAB2 antibody advances understanding of transcriptional repression, myelination, and tumorigenic signaling pathways.

UniProt: Q15742