RAD51D Antikörper (AA 23-328)

Produktnummer ABIN7872526

Der Kaninchen Polyklonal anti-RAD51D Antikörper (ABIN7872526) detektiert spezifisch RAD51D in WB, IHC, ELISA, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für RAD51D Antikörper (AA 23-328) (ABIN7872526)

Target: RAD51D (RAD51 Homolog D (RAD51D))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD51D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-RAD51D Antikörper

Bindungsspezifität: AA 23-328

Verwendungszweck: RAD51D Antibody / RAD51 homolog D

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RAD51D recombinant protein (Position: H23-T328) was used as the immunogen for the RAD51D antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RAD51D antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RAD51D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RAD51D

Target: RAD51D (RAD51 Homolog D (RAD51D))

Andere Bezeichnung: RAD51D

Hintergrund: RAD51D antibody detects DNA repair protein RAD51 homolog D, encoded by the RAD51D gene. RAD51D is a member of the RAD51 family of proteins that mediate homologous recombination, a high-fidelity DNA repair pathway essential for genome stability. RAD51D antibody provides researchers with a tool for studying DNA repair, recombination, and cancer susceptibility.

RAD51D functions by promoting strand invasion and pairing between homologous DNA sequences during double-strand break repair. Research using RAD51D antibody has shown that it forms complexes with other RAD51 paralogs, including RAD51B, RAD51C, and XRCC2, to stabilize DNA intermediates and ensure proper recombination. These interactions highlight RAD51D as a core mediator of genome maintenance.

Studies with RAD51D antibody have revealed that mutations in RAD51D predispose individuals to hereditary ovarian and breast cancer. Loss-of-function mutations compromise homologous recombination, leading to accumulation of DNA breaks and genomic instability. This genetic predisposition places RAD51D in the same pathway as BRCA1 and BRCA2, emphasizing its importance in hereditary cancer risk.

In addition to hereditary cancer, RAD51D has relevance in therapeutic contexts. Research using RAD51D antibody has demonstrated that cells deficient in RAD51D are highly sensitive to PARP inhibitors, which exploit synthetic lethality in DNA repair-deficient cancers. This makes RAD51D an important biomarker for precision oncology strategies.

RAD51D antibody is widely applied in western blotting, immunofluorescence, and chromatin immunoprecipitation. Western blotting quantifies expression in cell lines and tumor tissues, immunofluorescence highlights nuclear foci formation in response to DNA damage, and chromatin immunoprecipitation identifies DNA repair complexes. These methods make RAD51D antibody indispensable for DNA repair research.

By providing validated RAD51D antibody reagents, NSJ Bioreagents supports studies into DNA repair, hereditary cancer, and precision medicine. Detection of DNA repair protein RAD51 homolog D provides researchers with insight into how recombination pathways maintain genomic integrity.

UniProt: O75771