POMT1 Antikörper (AA 224-747)

Produktnummer ABIN7872396

Der Kaninchen Polyklonal anti-POMT1 Antikörper wird verwendet zum Nachweis von POMT1 in Proben von Human und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für POMT1 Antikörper (AA 224-747) (ABIN7872396)

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-POMT1 Antikörper

Bindungsspezifität: AA 224-747

Verwendungszweck: POMT1 Antibody / Protein O-mannosyl-transferase 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human POMT1 recombinant protein (Position: H224-H747) was used as the immunogen for the POMT1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the POMT1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the POMT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu POMT1

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Andere Bezeichnung: POMT1

Hintergrund: POMT1 antibody detects Protein O-mannosyl-transferase 1, an enzyme required for the initial step of O-mannosyl glycan biosynthesis on glycoproteins, particularly alpha-dystroglycan. The UniProt recommended name is Protein O-mannosyl-transferase 1 (POMT1). This enzyme catalyzes the transfer of mannose from dolichol phosphate-mannose to serine or threonine residues on target proteins, a process essential for proper protein folding, stability, and function.

Functionally, POMT1 antibody identifies a 747-amino-acid endoplasmic reticulum (ER) membrane protein that forms a functional heterodimer with POMT2. This complex initiates the O-mannosyl glycosylation pathway, which is critical for dystroglycan processing and muscle integrity. POMT1 ensures the attachment of the first mannose residue, forming the foundation for subsequent glycan extensions that link the extracellular matrix to the cytoskeleton via alpha-dystroglycan.

The POMT1 gene is located on chromosome 9q34.13 and is expressed in brain, skeletal muscle, and heart. It plays a vital role in muscle development, neuronal migration, and synaptic organization by supporting glycosylation-dependent cell adhesion and signaling.

Pathologically, mutations in POMT1 cause congenital muscular dystrophy-dystroglycanopathy type A1 (Walker-Warburg syndrome), type B1, and type C1. These disorders are characterized by muscular dystrophy, brain malformations, and ocular defects due to defective O-mannosylation of dystroglycan. Research using POMT1 antibody supports studies in glycosylation pathways, muscular dystrophy, and neuronal development.

POMT1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER glycosyltransferases. NSJ Bioreagents provides POMT1 antibody reagents optimized for studies in protein modification, muscle biology, and glycoprotein processing.

Structurally, Protein O-mannosyl-transferase 1 contains multiple transmembrane domains and a luminal catalytic domain harboring the conserved DXD motif required for glycosyltransferase activity. It assembles with POMT2 to form an enzymatically active complex that determines substrate recognition and catalysis. This antibody aids in investigating POMT1i 1/2s role in glycoprotein biosynthesis, muscle development, and congenital disease mechanisms.

UniProt: Q9Y6A1