TSR1 Antikörper (AA 224-723)

Produktnummer ABIN7872395

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TSR1 in ELISA, WB, FACS, IP, IF und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für TSR1 Antikörper (AA 224-723) (ABIN7872395)

Target: TSR1 (TSR1, 20S rRNA Accumulation, Homolog (TSR1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TSR1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TSR1 Antikörper

Bindungsspezifität: AA 224-723

Verwendungszweck: TSR1 Antibody / Pre-rRNA-processing protein TSR1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TSR1 recombinant protein (Position: D224-V723) was used as the immunogen for the TSR1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TSR1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TSR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TSR1

Target: TSR1 (TSR1, 20S rRNA Accumulation, Homolog (TSR1))

Andere Bezeichnung: TSR1

Hintergrund: TSR1 antibody detects Pre-rRNA-processing protein TSR1 homolog, a ribosome biogenesis factor required for the maturation of the small ribosomal subunit (40S). TSR1 is an essential nucleolar protein that participates in the late stages of pre-18S rRNA processing, ensuring proper ribosome assembly and translation efficiency. The TSR1 antibody is used in studies of ribosome formation, RNA metabolism, and cellular growth control.

TSR1 is encoded by the TSR1 gene located on human chromosome 17q25.3. The protein is approximately 88 kilodaltons and conserved across eukaryotes, underscoring its critical role in ribosome biogenesis. TSR1 associates with pre-ribosomal particles in the nucleolus and cytoplasm and functions during the transition of 90S to 40S ribosomal precursors. It acts as a structural scaffold that stabilizes pre-rRNA intermediates and recruits processing enzymes necessary for final 18S rRNA cleavage.

The TSR1 antibody typically detects an 88-90 kilodalton band on western blot and is useful for immunofluorescence studies showing nucleolar and cytoplasmic localization. Knockdown or mutation of TSR1 disrupts ribosome biogenesis, leading to impaired protein synthesis and activation of nucleolar stress pathways such as p53 stabilization. As a core assembly factor, TSR1 interacts with RIO kinases, NOB1, and other late-acting ribosome maturation proteins.

In addition to its canonical role in ribosome formation, TSR1 may contribute to cell cycle progression and proliferation through its control of global translation. Aberrant expression of TSR1 has been observed in several cancers, suggesting a link between elevated ribosome production and tumorigenesis. NSJ Bioreagents provides a validated TSR1 antibody, supporting detailed studies of ribosome assembly, nucleolar dynamics, and translation regulation.

UniProt: Q2NL82

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly