DCTPP1 Antikörper (AA 22-170)

Produktnummer ABIN7872285

Der Kaninchen Polyklonal anti-DCTPP1 Antikörper wird verwendet zum Nachweis von DCTPP1 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ELISA und FACS.

Kurzübersicht für DCTPP1 Antikörper (AA 22-170) (ABIN7872285)

Target: DCTPP1 (DCTP Pyrophosphatase 1 (DCTPP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCTPP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-DCTPP1 Antikörper

Bindungsspezifität: AA 22-170

Verwendungszweck: DCTPP1 Antibody / dCTP pyrophosphatase 1 / XTP3TPA

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human XTP3TPA/DCTPP1 recombinant protein (Position: R22-T170) was used as the immunogen for the DCTPP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the DCTPP1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the DCTPP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu DCTPP1

Target: DCTPP1 (DCTP Pyrophosphatase 1 (DCTPP1))

Andere Bezeichnung: DCTPP1

Hintergrund: DCTPP1 antibody detects dCTP pyrophosphatase 1 (also known as XTP3TPA), a nucleotide pool sanitizing enzyme that prevents the incorporation of damaged or modified nucleotides into DNA. Encoded by the DCTPP1 gene on chromosome 16p13.3, this enzyme hydrolyzes dCTP and its oxidized derivatives to their monophosphate forms, maintaining genomic integrity by reducing mutagenic nucleotide incorporation. DCTPP1 plays an essential role in DNA replication fidelity, repair, and protection against oxidative stress-induced mutagenesis.

Located primarily in the nucleus and mitochondria, DCTPP1 acts as a homotetrameric hydrolase with substrate specificity for dCTP and 5-methyl-dCTP. By regulating the deoxynucleotide pool, it prevents mispairing and epigenetic instability caused by cytosine modifications. Its activity supports accurate DNA replication and limits the formation of base substitution mutations. DCTPP1 expression increases under oxidative stress and in proliferating cells to safeguard replication accuracy.

The DCTPP1 antibody is widely used in DNA metabolism, cancer, and oxidative stress research to investigate nucleotide sanitization, genome maintenance, and replication control. Western blot analysis detects a 21 kilodalton band corresponding to DCTPP1, while immunofluorescence reveals both nuclear and mitochondrial localization depending on cell type. This antibody enables researchers to explore DNA repair dynamics and nucleotide pool balance in diverse biological systems.

Loss or dysregulation of DCTPP1 is associated with increased mutation rates, mitochondrial dysfunction, and genomic instability. Elevated expression has been observed in rapidly dividing tumor cells, where it supports proliferation by maintaining balanced nucleotide pools. The DCTPP1 antibody provides a powerful tool for studying DNA replication accuracy, oxidative defense, and cancer cell metabolism. NSJ Bioreagents supplies this antibody validated for its applications, ensuring consistent detection in studies of genome integrity and nucleotide metabolism.

UniProt: Q9H773

Pathways: SARS-CoV-2 Protein Interaktom