PTCD1 Antikörper (AA 203-601)

Produktnummer ABIN7872087

Der Kaninchen Polyklonal anti-PTCD1 Antikörper wird verwendet zum Nachweis von PTCD1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, FACS und IHC.

Kurzübersicht für PTCD1 Antikörper (AA 203-601) (ABIN7872087)

Target: PTCD1 (Pentatricopeptide Repeat Domain 1 (PTCD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTCD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Produktdetails anti-PTCD1 Antikörper

Bindungsspezifität: AA 203-601

Verwendungszweck: PTCD1 Antibody / Pentatricopeptide repeat domain-containing protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PTCD1 recombinant protein (Position: D203-L601) was used as the immunogen for the PTCD1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PTCD1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PTCD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PTCD1

Target: PTCD1 (Pentatricopeptide Repeat Domain 1 (PTCD1))

Andere Bezeichnung: PTCD1

Hintergrund: PTCD1 antibody detects Pentatricopeptide repeat domain-containing protein 1, a mitochondrial RNA-binding protein that regulates mitochondrial gene expression and RNA metabolism. Encoded by the PTCD1 gene on chromosome 2q31.3, this protein contains multiple tandem pentatricopeptide repeat (PPR) motifs that mediate specific binding to mitochondrial RNA transcripts. PTCD1 functions as a post-transcriptional regulator that controls the stability, processing, and translation of mitochondrial mRNAs essential for oxidative phosphorylation. By coordinating RNA maturation and ribosome assembly within mitochondria, PTCD1 ensures proper energy production and cellular respiration.

PTCD1 belongs to a large family of PPR proteins that are characterized by their modular RNA-binding repeats. In mammalian cells, PTCD1 localizes to the mitochondrial matrix and associates with mitochondrial ribonucleoprotein complexes. Functional studies indicate that PTCD1 modulates expression of key mitochondrial-encoded subunits of the respiratory chain, including components of complexes I and IV. Knockdown or loss of PTCD1 disrupts mitochondrial translation, leading to impaired ATP synthesis and increased reactive oxygen species production. As such, PTCD1 plays a central role in maintaining mitochondrial integrity and energy homeostasis.

The PTCD1 antibody is widely used in studies of mitochondrial biology, RNA processing, and metabolic regulation. In western blot assays, PTCD1 is detected as a ~75 kilodalton band, while immunofluorescence reveals punctate staining consistent with mitochondrial localization. Expression profiling has shown that PTCD1 is abundant in metabolically active tissues such as heart, skeletal muscle, and brain, where mitochondrial activity is high. The antibody is an essential reagent for examining mitochondrial gene expression pathways and for identifying alterations in mitochondrial function associated with metabolic disorders and neurodegeneration.

Beyond its core function in RNA metabolism, PTCD1 has been linked to mitochondrial-nuclear communication and apoptosis regulation. It may influence the transcription of nuclear-encoded mitochondrial genes by modulating signaling pathways that respond to mitochondrial dysfunction. Genetic variants of PTCD1 have been associated with susceptibility to metabolic diseases, including type 2 diabetes and mitochondrial encephalomyopathy. The PTCD1 antibody thus enables researchers to explore these regulatory networks and to define the molecular mechanisms that couple RNA metabolism with mitochondrial biogenesis. NSJ Bioreagents provides this antibody validated for its applications, supporting studies in bioenergetics, RNA regulation, and mitochondrial pathophysiology.

UniProt: O75127