XAF1 Antikörper (AA 197-301)

Produktnummer ABIN7871745

Der Kaninchen Polyklonal anti-XAF1 Antikörper wird verwendet zum Nachweis von XAF1 in Proben von Human. Er wurde validiert für WB, ELISA, IHC (p) und FACS.

Kurzübersicht für XAF1 Antikörper (AA 197-301) (ABIN7871745)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Produktdetails anti-XAF1 Antikörper

Bindungsspezifität: AA 197-301

Verwendungszweck: XIAP associated factor 1 Antibody / XAF1

Aufreinigung: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids Q197-S301) was used as the immunogen for the XIAP associated factor 1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the XIAP associated factor 1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the XIAP associated factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Andere Bezeichnung: XIAP associated factor 1

Hintergrund: XAF1, also called XIAP-associated factor 1, antagonizes the anti-caspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

UniProt: Q6GPH4