SRCIN1 Antikörper (AA 189-287)

Produktnummer ABIN7871525

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SRCIN1 in WB, ELISA, FACS und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für SRCIN1 Antikörper (AA 189-287) (ABIN7871525)

Target: SRCIN1 (SRC Kinase Signaling Inhibitor 1 (SRCIN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRCIN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SRCIN1 Antikörper

Bindungsspezifität: AA 189-287

Verwendungszweck: SRCIN1 Antibody

Aufreinigung: Antigen affinity purified

Immunogen: Amino acids E189-E287 from the human protein were used as the immunogen for the SRCIN1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SRCIN1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SRCIN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SRCIN1

Target: SRCIN1 (SRC Kinase Signaling Inhibitor 1 (SRCIN1))

Andere Bezeichnung: SRCIN1

Hintergrund: Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322). Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. Indirect immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.

UniProt: Q9C0H9