RECK Antikörper (AA 179-806)

Produktnummer ABIN7871358

Der Kaninchen Polyklonal anti-RECK Antikörper wird verwendet zum Nachweis von RECK in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für RECK Antikörper (AA 179-806) (ABIN7871358)

Target: RECK (Reversion-Inducing-Cysteine-Rich Protein with Kazal Motifs (RECK))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RECK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-RECK Antikörper

Bindungsspezifität: AA 179-806

Verwendungszweck: RECK Antibody / Reversion-inducing cysteine-rich protein with Kazal motifs

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RECK recombinant protein (Position: Q179-K806) was used as the immunogen for the RECK antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RECK antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RECK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RECK

Target: RECK (Reversion-Inducing-Cysteine-Rich Protein with Kazal Motifs (RECK))

Andere Bezeichnung: RECK

Hintergrund: RECK antibody detects Reversion-inducing cysteine-rich protein with Kazal motifs, a membrane-anchored glycoprotein that negatively regulates matrix metalloproteinases (MMPs) and suppresses tumor invasion and angiogenesis. The UniProt recommended name is Reversion-inducing cysteine-rich protein with Kazal motifs (RECK). This protein acts as an extracellular matrix (ECM) regulator that maintains tissue integrity and prevents metastasis by inhibiting proteolytic remodeling.

Functionally, RECK antibody identifies a 971-amino-acid glycosylphosphatidylinositol (GPI)-anchored protein expressed on the cell surface. RECK directly inhibits MMP2, MMP9, and MMP14, limiting ECM degradation and tumor cell invasion. It also stabilizes tissue inhibitors of metalloproteinases (TIMPs) and modulates Notch and Wnt signaling pathways involved in development and tumor suppression. RECK expression is tightly controlled by transcriptional regulators and epigenetic mechanisms, including promoter methylation and microRNA repression.

The RECK gene is located on chromosome 9p13.3 and is broadly expressed in embryonic and adult tissues, including brain, lung, and vascular endothelium. High expression is associated with normal tissue homeostasis and morphogenesis, whereas silencing occurs in many cancers, correlating with poor prognosis.

Pathologically, RECK downregulation promotes cancer metastasis, vascular invasion, and inflammation by unleashing matrix-degrading enzymes. Conversely, forced expression of RECK inhibits tumor angiogenesis and cell migration. RECK deficiency also impairs embryonic vascular development, reflecting its essential role in morphogenesis. Research using RECK antibody supports studies in oncology, extracellular matrix biology, and developmental signaling.

RECK antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect MMP regulators. NSJ Bioreagents provides RECK antibody reagents optimized for research in metastasis inhibition, angiogenesis, and extracellular matrix control.

Structurally, Reversion-inducing cysteine-rich protein with Kazal motifs contains multiple cysteine-rich and Kazal-type serine protease inhibitor domains that confer high affinity for MMP catalytic sites. Its GPI anchor tethers it to the plasma membrane, positioning RECK for local ECM modulation. This antibody enables exploration of RECK's tumor-suppressive mechanisms and its role in cell-matrix interactions.

UniProt: O95980