NFIA Antikörper (AA 172-233)

Produktnummer ABIN7871264

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NFIA in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NFIA Antikörper (AA 172-233) (ABIN7871264)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NFIA Antikörper

Bindungsspezifität: AA 172-233

Verwendungszweck: NFIA Antibody / Nuclear factor 1 A-type

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NFIA recombinant protein (Position: V172-Q233) was used as the immunogen for the NFIA antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NFIA antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NFIA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Andere Bezeichnung: NFIA

Hintergrund: NFIA antibody detects Nuclear factor 1 A-type, a member of the NFI transcription factor family involved in gene regulation during development, differentiation, and glial cell maturation. NFIA functions as both an activator and repressor of transcription and binds to the palindromic NFI recognition site present in numerous promoters. It plays critical roles in the development of the central nervous system, particularly in astrocyte differentiation, corpus callosum formation, and regulation of glial-specific gene expression. The NFIA antibody is a widely used tool for exploring transcriptional mechanisms that control neural development and glial identity.

NFIA is encoded by the NFIA gene located on human chromosome 1p31.3. The protein contains a conserved N-terminal DNA-binding domain responsible for dimerization and sequence-specific binding, along with a C-terminal transactivation/repression region that interacts with other transcriptional regulators. NFIA forms homo- and heterodimers with other NFI family members (NFIB, NFIC, NFIX), allowing combinatorial control of target genes. Studies in model organisms have shown that loss of NFIA disrupts gliogenesis, leading to abnormal brain structure and impaired glial differentiation.

Expression profiling reveals that NFIA is abundant in glial progenitor cells and astrocytes, while its expression declines as neurons mature. Western blot analysis using NFIA antibody typically identifies a band near 54-60 kDa (unphosphorylated form), corresponding to the full-length transcription factor. Immunohistochemistry reveals strong nuclear localization in developing brain regions, spinal cord, and other tissues undergoing differentiation. NFIA cooperates with SOX9, STAT3, and other transcriptional factors to regulate astrocytic gene programs, such as those encoding GFAP and S100B.

In addition to its role in neurodevelopment, NFIA participates in the regulation of kidney morphogenesis, adipogenesis, and hematopoietic stem cell maintenance. Dysregulation or mutation of NFIA has been linked to developmental delay, macrocephaly, and agenesis of the corpus callosum in human syndromic cases. NSJ Bioreagents provides a validated NFIA antibody optimized for western blot, immunohistochemistry, and chromatin studies, making it a key reagent for dissecting transcriptional networks in development and disease.

UniProt: Q12857