SETBP1 Antikörper (AA 172-1519)

Produktnummer ABIN7871262

Der Kaninchen Polyklonal anti-SETBP1 Antikörper wird verwendet zum Nachweis von SETBP1 in Proben von Human und Maus. Er wurde validiert für ELISA, WB, IF, FACS und ICC.

Kurzübersicht für SETBP1 Antikörper (AA 172-1519) (ABIN7871262)

Target: SETBP1 (SET Binding Protein 1 (SETBP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SETBP1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-SETBP1 Antikörper

Bindungsspezifität: AA 172-1519

Verwendungszweck: SETBP1 Antibody / SET binding protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SETBP1 recombinant protein (Position: S172-A1519) was used as the immunogen for the SETBP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SETBP1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SETBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SETBP1

Target: SETBP1 (SET Binding Protein 1 (SETBP1))

Andere Bezeichnung: SETBP1

Hintergrund: SETBP1 antibody detects SET binding protein 1, a nuclear protein that interacts with the SET oncoprotein to regulate chromatin structure, gene expression, and cellular proliferation. SETBP1 modulates transcriptional programs controlling stem cell maintenance and oncogenic transformation. The SETBP1 antibody is used in studies of leukemia, developmental syndromes, and transcriptional regulation.

SETBP1 is encoded by the SETBP1 gene on human chromosome 18q12.3. The protein is approximately 170 kilodaltons and contains multiple AT-hook motifs for DNA binding, nuclear localization signals, and a SET-binding domain that mediates interaction with the SET protein, a known inhibitor of protein phosphatase 2A (PP2A). Through this interaction, SETBP1 indirectly modulates PP2A activity, influencing cell cycle progression and epigenetic control.

The SETBP1 antibody detects a 145-150 kilodalton band by western blot and shows nuclear localization under immunofluorescence. SETBP1 acts as a transcriptional regulator that binds GC-rich promoter regions to enhance expression of target genes, including HOXA cluster members associated with hematopoietic differentiation and leukemogenesis. Mutations that stabilize SETBP1 lead to aberrant accumulation, transcriptional reprogramming, and malignant transformation in myelodysplastic syndromes and acute myeloid leukemia.

In addition to cancer, germline mutations in SETBP1 cause Schinzel-Giedion syndrome, a severe developmental disorder characterized by craniofacial abnormalities and neurological impairment. These mutations increase SETBP1 stability, disrupt normal transcriptional repression, and impair neuronal differentiation. SETBP1 also interacts with chromatin-modifying complexes to coordinate histone acetylation and methylation status.

As a molecular bridge between SET and chromatin, SETBP1 integrates signaling pathways that regulate proliferation, apoptosis, and gene expression. NSJ Bioreagents provides a validated SETBP1 antibody optimized for western blot, immunocytochemistry, and chromatin studies, facilitating research into oncogenic transcriptional regulation and epigenetic mechanisms in disease.

UniProt: Q9Y6X0