LSM11 Antikörper (AA 17-360)

Produktnummer ABIN7871228

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LSM11 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für LSM11 Antikörper (AA 17-360) (ABIN7871228)

Target: LSM11 (LSM11, U7 Small Nuclear RNA Associated (LSM11))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSM11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-LSM11 Antikörper

Bindungsspezifität: AA 17-360

Verwendungszweck: LSM11 Antibody / U7 snRNA-associated Sm-like protein LSm11

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LSM11 recombinant protein (Position: A17-Q360) was used as the immunogen for the LSM11 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LSM11 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LSM11 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LSM11

Target: LSM11 (LSM11, U7 Small Nuclear RNA Associated (LSM11))

Andere Bezeichnung: LSM11

Hintergrund: LSM11 antibody detects U7 small nuclear RNA-associated Sm-like protein LSM11, a nuclear RNA-binding protein essential for histone pre-mRNA processing. The UniProt recommended name is U7 small nuclear RNA-associated Sm-like protein LSM11 (LSM11). This protein functions as part of a specialized LSM complex that recognizes U7 snRNA, forming the U7 small nuclear ribonucleoprotein (snRNP) complex involved in 3'-end processing of replication-dependent histone transcripts.

Functionally, LSM11 antibody identifies a 383-amino-acid nuclear protein that partners with LSM10 and other U7 snRNP components to direct histone mRNA 3'-end cleavage. Unlike polyadenylated transcripts, replication-dependent histone mRNAs end in a conserved stem-loop followed by a purine-rich histone downstream element (HDE). The LSM11-containing U7 snRNP recognizes this sequence and recruits the histone cleavage complex to generate mature histone mRNA. This process ensures tight coupling of histone production to DNA replication during S phase.

The LSM11 gene is located on chromosome 5q35.3 and encodes a nuclear-localized protein expressed in proliferating cells, where histone synthesis is active. LSM11 contains Sm motifs that mediate RNA binding and protein-protein interactions essential for snRNP assembly. It directly interacts with FLASH (FLICE-associated huge protein) and the histone cleavage complex subunits to coordinate endonucleolytic processing of histone pre-mRNAs.

Beyond its canonical role in histone RNA processing, LSM11 contributes to transcriptional regulation and genome stability. It has been implicated in the activation of p53-dependent apoptosis under replication stress conditions through the U7 snRNP-FLASH pathway. By modulating histone synthesis, LSM11 indirectly affects chromatin assembly and DNA packaging during cell division. Perturbation of LSM11 expression leads to cell cycle arrest, defective chromatin condensation, and genomic instability.

LSM11 antibody is widely used in molecular biology, RNA processing, and cell cycle research. It is suitable for immunoblotting, immunofluorescence, and RNA-protein interaction studies to examine LSM11 localization and function. This antibody supports research into histone mRNA metabolism, snRNP assembly, and nuclear RNA processing mechanisms. In cancer and developmental studies, LSM11 detection aids in investigating how histone synthesis regulation impacts proliferation and differentiation.

Structurally, LSM11 shares the characteristic Sm-fold found in RNA-binding LSM proteins, forming ring-like complexes that interact with U7 snRNA. Its N-terminal region is important for FLASH recruitment, while the C-terminal domain stabilizes the U7 snRNP. NSJ Bioreagents provides LSM11 antibody reagents validated for use in RNA processing, histone biosynthesis, and nuclear complex research.

UniProt: P83369