TDO2 Antikörper (AA 16-406)

Produktnummer ABIN7871075

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TDO2 in WB, IHC, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für TDO2 Antikörper (AA 16-406) (ABIN7871075)

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-TDO2 Antikörper

Bindungsspezifität: AA 16-406

Verwendungszweck: TDO2 Antibody / Tryptophan 2,3-dioxygenase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TDO2 recombinant protein (Position: K16-D406) was used as the immunogen for the TDO2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TDO2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TDO2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TDO2

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Andere Bezeichnung: TDO2

Hintergrund: TDO2 antibody detects tryptophan 2,3-dioxygenase, a heme-dependent enzyme that catalyzes the first step of the kynurenine pathway, converting L-tryptophan to N-formylkynurenine. The UniProt recommended name is Tryptophan 2,3-dioxygenase (TDO2). This enzyme regulates systemic tryptophan levels and influences serotonin biosynthesis, immune tolerance, and NAD+ metabolism.

Functionally, TDO2 antibody identifies a homotetrameric enzyme that incorporates molecular oxygen into the indole ring of tryptophan. The enzyme is predominantly expressed in liver but is inducible in many tumor types. TDO2 activity shapes the immune microenvironment by depleting tryptophan and generating immunosuppressive kynurenine metabolites. This mechanism contributes to tumor immune evasion and cancer progression.

The TDO2 gene is located on chromosome 4q32.1 and encodes a 406-amino-acid protein with a conserved heme-binding pocket. Its expression is transcriptionally induced by glucocorticoids, stress, and pro-inflammatory cytokines. Elevated TDO2 levels have been reported in hepatocellular carcinoma, glioblastoma, and melanoma, positioning it as a therapeutic target in oncology. TDO2 also regulates neuroactive tryptophan metabolites implicated in mood disorders and neurodegeneration.

Pathologically, upregulation of TDO2 disturbs immune cell proliferation and antigen presentation through depletion of tryptophan and accumulation of kynurenine. Inhibiting TDO2 restores T-cell activity and is an emerging immunotherapeutic strategy complementary to checkpoint blockade. In metabolic studies, altered TDO2 activity links to hepatic inflammation and altered redox homeostasis.

TDO2 antibody is widely applied in cancer immunology, metabolism, and neurobiology research. It is suitable for immunoblotting, immunohistochemistry, and enzyme assays detecting TDO2 expression or localization. NSJ Bioreagents supplies high-quality TDO2 antibody reagents validated for use in relevant metabolic and immunological assays.

Structurally, tryptophan 2,3-dioxygenase possesses a catalytic heme group coordinated by a proximal histidine. Substrate binding induces conformational changes that enhance dioxygen activation. This antibody supports biochemical and translational studies targeting TDO2 for metabolic modulation and immunotherapy development.

UniProt: P48775