BOP1 Antikörper (AA 157-701)

Produktnummer ABIN7871024

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch BOP1 in WB, ELISA, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für BOP1 Antikörper (AA 157-701) (ABIN7871024)

Target: BOP1 (Block of Proliferation 1 (BOP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BOP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-BOP1 Antikörper

Bindungsspezifität: AA 157-701

Verwendungszweck: BOP1 Antibody / Block of proliferation 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human BOP1 recombinant protein (Position: R157-N701) was used as the immunogen for the BOP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the BOP1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the BOP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu BOP1

Target: BOP1 (Block of Proliferation 1 (BOP1))

Andere Bezeichnung: BOP1

Hintergrund: BOP1 antibody detects Block of proliferation 1 protein, a nucleolar factor that plays a central role in ribosome biogenesis, cell cycle regulation, and tumor progression. BOP1 forms part of the PeBoW complex (with PES1 and WDR12), which is required for maturation of the 28S and 5.8S ribosomal RNAs and assembly of the 60S ribosomal subunit. The BOP1 antibody is used in studies of ribosome formation, cellular proliferation, and oncogenic transformation.

BOP1 is encoded by the BOP1 gene located on human chromosome 8q24.3, a genomic region frequently amplified in cancers. The protein is approximately 80 kilodaltons and contains WD40 repeat motifs that mediate protein-protein interactions within the nucleolus. BOP1 functions as a structural scaffold coordinating pre-rRNA processing and ribonucleoprotein complex formation. Its proper activity is critical for balanced ribosome production and maintenance of genomic stability.

The BOP1 antibody detects a 100-110 kilodalton band by western blot and exhibits strong nucleolar localization by immunofluorescence. During ribosome assembly, BOP1 interacts with PES1 and WDR12 to stabilize preribosomal particles, ensuring efficient cleavage and processing of rRNA precursors. Loss of BOP1 results in accumulation of immature rRNA intermediates and nucleolar stress, leading to p53 activation and cell cycle arrest.

BOP1 also influences mitotic progression and centrosome duplication. Overexpression accelerates cell proliferation and promotes chromosomal instability, while depletion inhibits tumor cell growth. Dysregulation of BOP1 has been reported in colorectal, pancreatic, and hepatocellular carcinomas, correlating with poor prognosis and increased metastatic potential.

Because ribosome biogenesis is tightly linked to cell growth, BOP1 serves as a valuable biomarker for cancer diagnostics and as a potential target for anti-proliferative therapies. NSJ Bioreagents provides a validated BOP1 antibody optimized for western blot, immunofluorescence, and nucleolar imaging, supporting research into ribosomal RNA processing, nucleolar stress response, and oncogenic signaling pathways.

UniProt: Q14137