PDPR Antikörper (AA 155-655)

Produktnummer ABIN7871012

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PDPR in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für PDPR Antikörper (AA 155-655) (ABIN7871012)

Target: PDPR (Pyruvate Dehydrogenase Phosphatase Regulatory Subunit (PDPR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PDPR Antikörper

Bindungsspezifität: AA 155-655

Verwendungszweck: PDPR Antibody / Pyruvate dehydrogenase phosphatase regulatory subunit

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PDPR recombinant protein (Position: E155-E655) was used as the immunogen for the PDPR antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PDPR antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PDPR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PDPR

Target: PDPR (Pyruvate Dehydrogenase Phosphatase Regulatory Subunit (PDPR))

Andere Bezeichnung: PDPR

Hintergrund: PDPR antibody detects Pyruvate dehydrogenase phosphatase regulatory subunit, a mitochondrial matrix protein encoded by the PDPR gene located on chromosome 16q22.1. PDPR acts as a regulatory subunit of the pyruvate dehydrogenase phosphatase (PDP) complex, which controls activity of the pyruvate dehydrogenase complex (PDC) - a critical enzyme in carbohydrate metabolism that links glycolysis to the tricarboxylic acid (TCA) cycle. PDPR is highly expressed in metabolically active tissues such as liver, heart, skeletal muscle, and kidney, where it modulates energy production and metabolic flexibility.

PDPR functions by modulating the catalytic activity of PDP1 and PDP2 phosphatase subunits, facilitating dephosphorylation and reactivation of PDC. This regulation ensures efficient conversion of pyruvate to acetyl-CoA, thereby supporting ATP generation under nutrient-rich conditions. PDPR also influences glucose oxidation rates and metabolic adaptation during fasting, exercise, or insulin stimulation. Co-localization studies show PDPR associating with PDC and PDP1 in the mitochondrial matrix, maintaining dynamic control over carbohydrate flux.

Structurally, PDPR contains an N-terminal domain involved in protein-protein interactions and a C-terminal alpha-helical region required for complex stability. It belongs to the pyruvate dehydrogenase regulatory protein family, which fine-tunes mitochondrial energy metabolism. The proteini 1/2s structural motifs enable binding with PDP catalytic subunits and coordination with mitochondrial targeting sequences for correct localization.

Functionally, PDPR integrates metabolic and hormonal cues to maintain energy homeostasis. It plays a vital role in glucose utilization, mitochondrial respiration, and lipid oxidation balance. PDPR is regulated by insulin, glucagon, and nutrient availability, allowing cells to rapidly switch between carbohydrate and fatty acid metabolism. During embryonic development, PDPR supports energy demands of rapidly growing tissues, particularly cardiac and skeletal muscle. Pathway involvement includes the TCA cycle, oxidative phosphorylation, and insulin signaling pathways that coordinate energy metabolism.

Dysregulation of PDPR activity is linked to metabolic diseases, including diabetes, obesity, and mitochondrial disorders. Reduced PDPR function leads to impaired PDC activation, resulting in accumulation of pyruvate and lactic acid, while overactivation can alter glucose oxidation balance. Genetic variants in PDPR have been associated with altered fasting glucose levels and insulin sensitivity. Because of its role in mitochondrial regulation, PDPR has also been studied in cancer metabolism, where metabolic reprogramming supports tumor cell proliferation.

Immunohistochemical staining using PDPR antibody shows mitochondrial localization in liver, cardiac, and muscle cells. The PDPR antibody from NSJ Bioreagents is a valuable reagent for research on mitochondrial metabolism, energy regulation, and metabolic disease mechanisms.

UniProt: Q8NCN5