MEIOB Antikörper (AA 140-442)

Produktnummer ABIN7870665

Der Kaninchen Polyklonal anti-MEIOB Antikörper wird verwendet zum Nachweis von MEIOB in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, FACS, ICC und IF.

Kurzübersicht für MEIOB Antikörper (AA 140-442) (ABIN7870665)

Target: MEIOB (Meiosis Specific with OB Domains (MEIOB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEIOB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-MEIOB Antikörper

Bindungsspezifität: AA 140-442

Verwendungszweck: MEIOB Antibody / Meiosis-specific with OB domains protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MEIOB recombinant protein (Position: I140-V442) was used as the immunogen for the MEIOB antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MEIOB antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MEIOB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MEIOB

Target: MEIOB (Meiosis Specific with OB Domains (MEIOB))

Andere Bezeichnung: MEIOB

Hintergrund: MEIOB antibody detects Meiosis-specific with OB domains protein, a single-stranded DNA-binding protein essential for homologous recombination and meiotic crossover formation. The UniProt recommended name is Meiosis-specific with OB domains protein (MEIOB). This nuclear factor participates in the repair of programmed DNA double-strand breaks (DSBs) during meiosis, ensuring accurate chromosomal segregation and gamete genomic integrity.

Functionally, MEIOB antibody identifies a 471-amino-acid protein that localizes to meiotic chromatin in germ cells. MEIOB interacts with single-stranded DNA at resected DSBs and forms a complex with SPATA22, which stabilizes the DNA repair intermediate and promotes strand exchange facilitated by recombinases DMC1 and RAD51. Together, these interactions drive the homologous recombination events that generate genetic diversity during gametogenesis.

The MEIOB gene is located on chromosome 16p13.3 and is expressed exclusively in meiotic cells of the testis and ovary. MEIOB contains oligonucleotide/oligosaccharide-binding (OB) folds characteristic of single-stranded DNA-binding proteins. Its expression begins at the leptotene stage of meiosis and persists through pachytene, where it coordinates crossover formation and synapsis of homologous chromosomes. Loss of MEIOB function results in defective recombination, impaired synaptonemal complex formation, and meiotic arrest.

In mammals, MEIOB plays an indispensable role in meiotic DSB repair by stabilizing single-stranded DNA intermediates and promoting strand invasion. It also prevents excessive nuclease activity that could lead to genome instability. In humans, MEIOB mutations have been linked to infertility due to defective gametogenesis, emphasizing its essential contribution to reproductive success. In addition to its germline function, MEIOB expression may serve as a biomarker for spermatogenic activity and ovarian follicle development.

MEIOB antibody is widely used in reproductive biology, genetics, and DNA repair research. It is suitable for immunohistochemistry, immunoblotting, and chromatin association studies to track MEIOB localization and complex formation. This antibody supports investigations into meiotic recombination, chromosomal synapsis, and genetic stability. It is also used in infertility research to assess meiotic defects and germ cell differentiation.

Structurally, MEIOB possesses two OB-fold domains for DNA binding and forms a stoichiometric complex with SPATA22 that mimics the RPA-like architecture. NSJ Bioreagents provides MEIOB antibody reagents validated for use in meiosis, recombination, and fertility research.

UniProt: Q8N635