NAGK Antikörper (AA 14-261)

Produktnummer ABIN7870623

Der Kaninchen Polyklonal anti-NAGK Antikörper wird verwendet zum Nachweis von NAGK in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und FACS.

Kurzübersicht für NAGK Antikörper (AA 14-261) (ABIN7870623)

Target: NAGK (N-Acetylglucosamine Kinase (NAGK))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAGK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-NAGK Antikörper

Bindungsspezifität: AA 14-261

Verwendungszweck: NAGK Antibody / N-acetyl-D-glucosamine kinase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NAGK recombinant protein (Position: R14-K261) was used as the immunogen for the NAGK antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NAGK antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NAGK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NAGK

Target: NAGK (N-Acetylglucosamine Kinase (NAGK))

Andere Bezeichnung: NAGK

Hintergrund: NAGK antibody detects N-acetyl-D-glucosamine kinase, encoded by the NAGK gene on chromosome 2p13.3. NAGK antibody is widely used in metabolism, glycosylation, and signaling research. NAGK is an enzyme of the hexosamine biosynthetic pathway that phosphorylates N-acetylglucosamine (GlcNAc) to GlcNAc-6-phosphate. This reaction contributes to the salvage pathway of UDP-GlcNAc biosynthesis, supporting protein glycosylation, glycosaminoglycan synthesis, and O-GlcNAc signaling.

Structurally, NAGK is a ~37 kDa cytoplasmic protein belonging to the sugar kinase family. It forms homodimers and contains an ATP-binding site and catalytic residues conserved among carbohydrate kinases. NAGK is broadly expressed across tissues, reflecting its role in basic carbohydrate metabolism.

Functionally, NAGK channels free GlcNAc into UDP-GlcNAc production, which is required for N- and O-linked glycosylation of proteins and for biosynthesis of glycolipids and proteoglycans. It plays a critical role in cell growth, signaling, and metabolic adaptation. Researchers use NAGK antibody to study carbohydrate metabolism, glycosylation pathways, and metabolic regulation.

Clinically, altered NAGK activity is associated with metabolic diseases and cancer. Increased NAGK expression has been reported in certain tumors, where it supports growth by boosting glycosylation and nutrient sensing pathways. Because protein glycosylation is a therapeutic target, NAGK is under investigation as a biomarker and regulator of cancer metabolism. NSJ Bioreagents provides NAGK antibody for metabolism and glycosylation research.

Experimentally, NAGK antibody is used in western blotting to detect the ~37 kDa enzyme, in immunohistochemistry to study tissue distribution, and in immunofluorescence to confirm cytoplasmic localization. Enzyme assays with NAGK antibody complement biochemical analyses of hexosamine pathway flux.

UniProt: Q9UJ70