NOL9 Antikörper (AA 131-636)

Produktnummer ABIN7870509

Der Kaninchen Polyklonal anti-NOL9 Antikörper wird verwendet zum Nachweis von NOL9 in Proben von Human. Er wurde validiert für WB, ELISA, FACS, ICC und IF.

Kurzübersicht für NOL9 Antikörper (AA 131-636) (ABIN7870509)

Target: NOL9 (Nucleolar Protein 9 (NOL9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOL9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-NOL9 Antikörper

Bindungsspezifität: AA 131-636

Verwendungszweck: NOL9 Antibody / Nucleolar protein 9

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NOL9 recombinant protein (Position: E131-N636) was used as the immunogen for the NOL9 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NOL9 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NOL9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NOL9

Target: NOL9 (Nucleolar Protein 9 (NOL9))

Andere Bezeichnung: NOL9

Hintergrund: The NOL9 antibody targets Nucleolar protein 9, a multifunctional nucleolar protein encoded by the NOL9 gene. Nucleolar protein 9 participates in ribosomal RNA (rRNA) processing, specifically catalyzing the 5'-end maturation of 28S and 5.8S rRNA during large subunit biogenesis. It possesses polynucleotide 5'-kinase activity that promotes efficient pre-rRNA cleavage and processing. The NOL9 antibody enables researchers to study rRNA maturation, nucleolar organization, and ribosome assembly in proliferating cells.

Nucleolar protein 9 localizes to the granular component of the nucleolus, where late steps of ribosome biogenesis occur. It interacts with the Las1L complex, which couples endonucleolytic cleavage with phosphorylation of the pre-rRNA substrate. The NOL9 antibody supports detailed localization studies, showing characteristic punctate nucleolar staining consistent with ribosome assembly regions. Depletion of NOL9 disrupts pre-rRNA maturation and impairs large ribosomal subunit formation, leading to nucleolar stress and p53 activation.

Beyond ribosome biogenesis, Nucleolar protein 9 has been linked to cell growth regulation and stress responses. It participates in pathways that maintain nucleolar integrity under conditions of transcriptional or metabolic stress. The NOL9 antibody allows researchers to assess these processes and to investigate how nucleolar function integrates with global gene-expression control. Its activity is upregulated during proliferation, reflecting the dependence of ribosome production on cellular growth signals.

Disruption or downregulation of NOL9 has been observed in cancer and developmental disorders associated with defective ribosome synthesis. The NOL9 antibody provides a tool for analyzing expression patterns in tumor and normal tissues, supporting exploration of ribosome biogenesis as a therapeutic target. Elevated NOL9 expression correlates with high ribosomal demand in rapidly dividing cells, making it a marker of proliferative capacity.

The NOL9 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nucleolar localization. NSJ Bioreagents provides this antibody with verified specificity and reliability for use in ribosome biogenesis, nucleolar biology, and molecular cell research. By enabling accurate detection of Nucleolar protein 9, the NOL9 antibody advances understanding of rRNA processing, ribosomal maturation, and the cellular mechanisms that link nucleolar activity to growth and disease.

UniProt: Q5SY16