SESN3 Antikörper (AA 13-345)

Produktnummer ABIN7870470

Der Kaninchen Polyklonal anti-SESN3 Antikörper wird verwendet zum Nachweis von SESN3 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF, ICC und FACS.

Kurzübersicht für SESN3 Antikörper (AA 13-345) (ABIN7870470)

Target: SESN3 (Sestrin 3 (SESN3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SESN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-SESN3 Antikörper

Bindungsspezifität: AA 13-345

Verwendungszweck: SESN3 Antibody / Sestrin 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SESN3 recombinant protein (Position: N13-H345) was used as the immunogen for the SESN3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SESN3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SESN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SESN3

Target: SESN3 (Sestrin 3 (SESN3))

Andere Bezeichnung: SESN3

Hintergrund: The SESN3 antibody targets Sestrin-3, a stress-responsive protein encoded by the SESN3 gene that acts as a key regulator of metabolism, oxidative stress, and autophagy. Sestrin-3 belongs to the sestrin family of highly conserved proteins that modulate AMPK and mTORC1 signaling in response to cellular stress and nutrient availability. By integrating metabolic and redox cues, Sestrin-3 protects cells from oxidative damage and supports energy balance. The SESN3 antibody provides a high-specificity reagent for studying these protective pathways and their dysregulation in metabolic and age-related diseases.

Sestrin-3 functions as both an antioxidant and a signaling adaptor. It promotes activation of AMP-activated protein kinase (AMPK) and inhibits mechanistic target of rapamycin complex 1 (mTORC1), thereby promoting autophagy and metabolic homeostasis under nutrient stress. The SESN3 antibody enables detection of this adaptive protein across a range of tissues, including liver, muscle, adipose, and brain, where it contributes to maintaining glucose and lipid metabolism. Through these pathways, Sestrin-3 serves as a critical mediator of cellular recovery following oxidative or genotoxic stress.

In metabolic research, Sestrin-3 has gained prominence as a regulator of insulin sensitivity and lipid catabolism. Elevated SESN3 expression improves glucose tolerance and suppresses lipogenesis through AMPK activation. Conversely, its loss results in impaired energy metabolism and increased susceptibility to metabolic syndrome. The SESN3 antibody supports studies of these mechanisms by quantifying protein levels in metabolic tissues and cell lines subjected to nutrient deprivation or stress stimuli.

Sestrin-3 also contributes to antioxidant defense by reducing reactive oxygen species (ROS) accumulation and supporting regeneration of peroxiredoxins. These functions are critical in preventing oxidative stressi 1/2induced damage in aging, cardiovascular disease, and neurodegeneration. The SESN3 antibody allows detailed analysis of these processes and helps map signaling interactions with FOXO and p53 transcription factors that drive stress-response gene expression. Dysregulation of Sestrin-3 has been implicated in tumor progression, where its expression may modulate cell survival under metabolic stress conditions common in cancer microenvironments.

The SESN3 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence. It provides clear cytoplasmic staining consistent with the localization of this stress-inducible regulator. NSJ Bioreagents offers the SESN3 antibody as a validated reagent for reproducible detection across research platforms. By supporting detailed exploration of Sestrin-3 function in redox balance, energy metabolism, and autophagy, the SESN3 antibody advances understanding of cellular adaptation mechanisms that maintain metabolic health and longevity.

UniProt: P58005